Characterization of Maltase and Sucrase Inhibitory Constituents from <i>Rhodiola crenulata</i>
The inhibitory properties of epicatechin-(4<i>β</i>,8)-epicatechingallate (B2-3’<i>-O-</i>gallate), epicatechin gallate (ECG), and epicatechin (EC) isolated from <i>Rhodiola crenulata</i> toward maltase and sucrase were investigated. The half-ma...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/8/11/540 |
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| author | Wen-Tai Li Yu-Hsuan Chuang Jung-Feng Hsieh |
| author_facet | Wen-Tai Li Yu-Hsuan Chuang Jung-Feng Hsieh |
| author_sort | Wen-Tai Li |
| collection | DOAJ |
| description | The inhibitory properties of epicatechin-(4<i>β</i>,8)-epicatechingallate (B2-3’<i>-O-</i>gallate), epicatechin gallate (ECG), and epicatechin (EC) isolated from <i>Rhodiola crenulata</i> toward maltase and sucrase were investigated. The half-maximal inhibitory concentration (IC<sub>50</sub>) values for maltase were as follows: B2-3’-<i>O</i>-gallate (1.73 ± 1.37 μM), ECG (3.64 ± 2.99 μM), and EC (6.25 ± 1.84 μM). Inhibition kinetic assays revealed the inhibition constants (<i>K</i>i) of the mixed-competitive inhibitors of maltase, as follows: B2-3’-<i>O</i>-gallate (1.99 ± 0.02 μM), ECG (3.14 ± 0.04 μM), and EC (7.02 ± 0.26 μM). These compounds also showed a strong inhibitory activity toward sucrase, and the IC<sub>50</sub> values of B2-3’-<i>O</i>-gallate, ECG, and EC were 6.91 ± 3.41, 18.27 ± 3.99, and 18.91 ± 3.66 μM, respectively. Inhibition kinetic assays revealed the inhibition constants (<i>K</i>i) of the mixed-competitive inhibitors of sucrase as follows: B2-3’-<i>O</i>-gallate (6.05 ± 0.04 μM), ECG (8.58 ± 0.08 μM), and EC (13.72 ± 0.15 μM). Overall, these results suggest that B2-3’-<i>O</i>-gallate, ECG, and EC are potent maltase and sucrase inhibitors. |
| first_indexed | 2024-12-14T12:52:19Z |
| format | Article |
| id | doaj.art-486a7b75dd4a457d8292c24d03d78a3b |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-12-14T12:52:19Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-486a7b75dd4a457d8292c24d03d78a3b2022-12-21T23:00:38ZengMDPI AGFoods2304-81582019-11-0181154010.3390/foods8110540foods8110540Characterization of Maltase and Sucrase Inhibitory Constituents from <i>Rhodiola crenulata</i>Wen-Tai Li0Yu-Hsuan Chuang1Jung-Feng Hsieh2National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 11221, TaiwanDepartment of Food Science, Fu Jen Catholic University, Taipei 242, TaiwanDepartment of Food Science, Fu Jen Catholic University, Taipei 242, TaiwanThe inhibitory properties of epicatechin-(4<i>β</i>,8)-epicatechingallate (B2-3’<i>-O-</i>gallate), epicatechin gallate (ECG), and epicatechin (EC) isolated from <i>Rhodiola crenulata</i> toward maltase and sucrase were investigated. The half-maximal inhibitory concentration (IC<sub>50</sub>) values for maltase were as follows: B2-3’-<i>O</i>-gallate (1.73 ± 1.37 μM), ECG (3.64 ± 2.99 μM), and EC (6.25 ± 1.84 μM). Inhibition kinetic assays revealed the inhibition constants (<i>K</i>i) of the mixed-competitive inhibitors of maltase, as follows: B2-3’-<i>O</i>-gallate (1.99 ± 0.02 μM), ECG (3.14 ± 0.04 μM), and EC (7.02 ± 0.26 μM). These compounds also showed a strong inhibitory activity toward sucrase, and the IC<sub>50</sub> values of B2-3’-<i>O</i>-gallate, ECG, and EC were 6.91 ± 3.41, 18.27 ± 3.99, and 18.91 ± 3.66 μM, respectively. Inhibition kinetic assays revealed the inhibition constants (<i>K</i>i) of the mixed-competitive inhibitors of sucrase as follows: B2-3’-<i>O</i>-gallate (6.05 ± 0.04 μM), ECG (8.58 ± 0.08 μM), and EC (13.72 ± 0.15 μM). Overall, these results suggest that B2-3’-<i>O</i>-gallate, ECG, and EC are potent maltase and sucrase inhibitors.https://www.mdpi.com/2304-8158/8/11/540maltasesucraseinhibitorinhibition kinetics assay<i>rhodiola crenulata</i> |
| spellingShingle | Wen-Tai Li Yu-Hsuan Chuang Jung-Feng Hsieh Characterization of Maltase and Sucrase Inhibitory Constituents from <i>Rhodiola crenulata</i> Foods maltase sucrase inhibitor inhibition kinetics assay <i>rhodiola crenulata</i> |
| title | Characterization of Maltase and Sucrase Inhibitory Constituents from <i>Rhodiola crenulata</i> |
| title_full | Characterization of Maltase and Sucrase Inhibitory Constituents from <i>Rhodiola crenulata</i> |
| title_fullStr | Characterization of Maltase and Sucrase Inhibitory Constituents from <i>Rhodiola crenulata</i> |
| title_full_unstemmed | Characterization of Maltase and Sucrase Inhibitory Constituents from <i>Rhodiola crenulata</i> |
| title_short | Characterization of Maltase and Sucrase Inhibitory Constituents from <i>Rhodiola crenulata</i> |
| title_sort | characterization of maltase and sucrase inhibitory constituents from i rhodiola crenulata i |
| topic | maltase sucrase inhibitor inhibition kinetics assay <i>rhodiola crenulata</i> |
| url | https://www.mdpi.com/2304-8158/8/11/540 |
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