Antioxidant Peptides from <i>Sepia esculenta</i> Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in <i>Caenorhabditis elegans</i>
The hydrolysate of golden cuttlefish (<i>Sepia esculenta</i>) was prepared by using papain, and then, it was further separated by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC). The peptide components of the active fract...
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MDPI AG
2020-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/10/490 |
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| author | Xuesong Yu Qina Su Tianqi Shen Qiong Chen Ying Wang Weizhang Jia |
| author_facet | Xuesong Yu Qina Su Tianqi Shen Qiong Chen Ying Wang Weizhang Jia |
| author_sort | Xuesong Yu |
| collection | DOAJ |
| description | The hydrolysate of golden cuttlefish (<i>Sepia esculenta</i>) was prepared by using papain, and then, it was further separated by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC). The peptide components of the active fraction were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and then two novel peptides, SeP2 (DVEDLEAGLAK, 1159.27 Da) and SeP5 (EITSLAPSTM, 1049.22 Da), were obtained and displayed significant alleviation effects on oxidative stress in <i>Caenorhabditis elegans</i>. Studies indicated that <i>S. esculenta</i> antioxidant peptides (SePs) increase superoxide dismutase (SOD) activity but reduce reactive oxygen species (ROS) and malondialdehyde (MDA) levelsin oxidation-damaged nematodes. Using transgenic CF1553 nematodes, the <i>sod-3p</i>::GFP expression in the worms treated with SePs was significantly higher than that of the control nematodes. Real-time PCR also demonstrated that the expression of stress-related genes such as <i>sod-3</i> is up-regulated by SePs. Furthermore, studies showed that SePs could obviously decrease fat accumulation as well as reduce the elevated ROS and MDA levels in high-fat nematodes. Taken together, these results indicated that SePs are capable of the activation of antioxidant defense and the inhibition of free radicals and lipid peroxidation, play important roles in attenuating oxidative stress and fat accumulation in <i>C. elegans</i>, and might have the potential to be used in nutraceutical and functional foods. |
| first_indexed | 2024-03-10T16:02:35Z |
| format | Article |
| id | doaj.art-4872c333156f44818fc0625a8f9456f4 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T16:02:35Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-4872c333156f44818fc0625a8f9456f42023-11-20T15:10:31ZengMDPI AGMarine Drugs1660-33972020-09-01181049010.3390/md18100490Antioxidant Peptides from <i>Sepia esculenta</i> Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in <i>Caenorhabditis elegans</i>Xuesong Yu0Qina Su1Tianqi Shen2Qiong Chen3Ying Wang4Weizhang Jia5School of Biosciences & Biopharmaceutics and Guangdong Province Key Laboratory for Biotehnology Drug Candidates, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Biosciences & Biopharmaceutics and Guangdong Province Key Laboratory for Biotehnology Drug Candidates, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Biosciences & Biopharmaceutics and Guangdong Province Key Laboratory for Biotehnology Drug Candidates, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Biosciences & Biopharmaceutics and Guangdong Province Key Laboratory for Biotehnology Drug Candidates, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaInstitutes for Life Sciences and School of Medicine, South China University of Technology, Guangzhou 510641, ChinaSchool of Biosciences & Biopharmaceutics and Guangdong Province Key Laboratory for Biotehnology Drug Candidates, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaThe hydrolysate of golden cuttlefish (<i>Sepia esculenta</i>) was prepared by using papain, and then, it was further separated by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC). The peptide components of the active fraction were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and then two novel peptides, SeP2 (DVEDLEAGLAK, 1159.27 Da) and SeP5 (EITSLAPSTM, 1049.22 Da), were obtained and displayed significant alleviation effects on oxidative stress in <i>Caenorhabditis elegans</i>. Studies indicated that <i>S. esculenta</i> antioxidant peptides (SePs) increase superoxide dismutase (SOD) activity but reduce reactive oxygen species (ROS) and malondialdehyde (MDA) levelsin oxidation-damaged nematodes. Using transgenic CF1553 nematodes, the <i>sod-3p</i>::GFP expression in the worms treated with SePs was significantly higher than that of the control nematodes. Real-time PCR also demonstrated that the expression of stress-related genes such as <i>sod-3</i> is up-regulated by SePs. Furthermore, studies showed that SePs could obviously decrease fat accumulation as well as reduce the elevated ROS and MDA levels in high-fat nematodes. Taken together, these results indicated that SePs are capable of the activation of antioxidant defense and the inhibition of free radicals and lipid peroxidation, play important roles in attenuating oxidative stress and fat accumulation in <i>C. elegans</i>, and might have the potential to be used in nutraceutical and functional foods.https://www.mdpi.com/1660-3397/18/10/490antioxidant peptidesoxidative stressfat accumulation<i>Sepia esculenta</i><i>Caenorhabditis elegans</i> |
| spellingShingle | Xuesong Yu Qina Su Tianqi Shen Qiong Chen Ying Wang Weizhang Jia Antioxidant Peptides from <i>Sepia esculenta</i> Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in <i>Caenorhabditis elegans</i> Marine Drugs antioxidant peptides oxidative stress fat accumulation <i>Sepia esculenta</i> <i>Caenorhabditis elegans</i> |
| title | Antioxidant Peptides from <i>Sepia esculenta</i> Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in <i>Caenorhabditis elegans</i> |
| title_full | Antioxidant Peptides from <i>Sepia esculenta</i> Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in <i>Caenorhabditis elegans</i> |
| title_fullStr | Antioxidant Peptides from <i>Sepia esculenta</i> Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | Antioxidant Peptides from <i>Sepia esculenta</i> Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in <i>Caenorhabditis elegans</i> |
| title_short | Antioxidant Peptides from <i>Sepia esculenta</i> Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in <i>Caenorhabditis elegans</i> |
| title_sort | antioxidant peptides from i sepia esculenta i hydrolyzate attenuate oxidative stress and fat accumulation in i caenorhabditis elegans i |
| topic | antioxidant peptides oxidative stress fat accumulation <i>Sepia esculenta</i> <i>Caenorhabditis elegans</i> |
| url | https://www.mdpi.com/1660-3397/18/10/490 |
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