Quantification and characterization of mouse and human tissue-resident microbiota by qPCR and 16S sequencing

Summary: The tissue-resident microbiota is an integral component of multiple tumor types, but it remains challenging to characterize its abundance and composition due to its low biomass. Here, we describe an optimized protocol for quantification and profiling of tissue-resident microbiota. The major...

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Bibliographic Details
Main Authors: Bingqing Yao, Tingting Dong, Aikun Fu, Hang Li, Chuhan Jiang, Nan Li, Shang Cai
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006451