Quantification and characterization of mouse and human tissue-resident microbiota by qPCR and 16S sequencing
Summary: The tissue-resident microbiota is an integral component of multiple tumor types, but it remains challenging to characterize its abundance and composition due to its low biomass. Here, we describe an optimized protocol for quantification and profiling of tissue-resident microbiota. The major...
Main Authors: | Bingqing Yao, Tingting Dong, Aikun Fu, Hang Li, Chuhan Jiang, Nan Li, Shang Cai |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2022-12-01
|
Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006451 |
Similar Items
-
Innovative microRNA quantification by qPCR
by: Minh Ngoc Le, et al.
Published: (2023-03-01) -
Taxonomy annotation and guide tree errors in 16S rRNA databases
by: Robert Edgar
Published: (2018-06-01) -
Quantification of siRNA using competitive qPCR.
by: Liu, W, et al.
Published: (2009) -
qPCR
by: Lynne Lederman
Published: (2009-10-01) -
Rapidly quantification of intact infectious H1N1 virus using ICA-qPCR and PMA-qPCR
by: Chudan Liang, et al.
Published: (2024-12-01)