Detecting RNA-protein proximity at DNA double-strand breaks using combined fluorescence in situ hybridization with proximity ligation assay

Detecting RNA-protein proximity at DNA double-strand breaks using combined fluorescence in situ hybridization with proximity ligation assay

Summary: RNA transcribed at DNA double-strand breaks (DSBs) contributes to accurate DNA repair. Here, using the repair factors 53BP1 and TIRR as examples, we combine the fluorescence in situ hybridization (FISH) and proximity ligation assay (PLA) techniques to determine protein proximity to DSB-tran...

Full description

Bibliographic Details
Main Authors: Adele Alagia, Ruth F. Ketley, Monika Gullerova
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Microscopy
Molecular Biology
In Situ Hybridization
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000540

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723000540

Similar Items