Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency

Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency

The advantages of lipid-based transfection reagents have permitted their widespread use in molecular biology and gene therapy. This study outlines the effect of cryo-manipulation of a cationic lipid-based formulation, Lipofectamine 2000, which, after being frozen and thawed, showed orders of magnitu...

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Bibliographic Details
Main Authors: Helena Sork, Joel Z Nordin, Janne J Turunen, Oscar PB Wiklander, Burcu Bestas, Eman M Zaghloul, Helerin Margus, Kärt Padari, Adil D Duru, Giulia Corso, Jeremy Bost, Pieter Vader, Margus Pooga, CI Edvard Smith, Matthew JA Wood, Raymond M Schiffelers, Mattias Hällbrink, Samir EL Andaloussi
Format: Article
Language:English
Published: Elsevier 2016-01-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
freezing
Lipofectamine 2000
lipofection
lipoplex
transfection
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253117301099

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http://www.sciencedirect.com/science/article/pii/S2162253117301099

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