HPTLC and ATR/FTIR Characterization of Antioxidants in Different Rosemary Extracts
The effect of spontaneous fermentation by lactic acid bacteria on the extraction yield of bioactive compounds and antioxidant activity from rosemary leaf extracts was investigated using high-performance thin-layer chromatography (HPTLC). Brining and spontaneous fermentation with lactic acid bacteria...
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MDPI AG
2021-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/19/6064 |
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| author | Snezana Agatonovic-Kustrin Ksenia S. Balyklova Vladimir Gegechkori David W. Morton |
| author_facet | Snezana Agatonovic-Kustrin Ksenia S. Balyklova Vladimir Gegechkori David W. Morton |
| author_sort | Snezana Agatonovic-Kustrin |
| collection | DOAJ |
| description | The effect of spontaneous fermentation by lactic acid bacteria on the extraction yield of bioactive compounds and antioxidant activity from rosemary leaf extracts was investigated using high-performance thin-layer chromatography (HPTLC). Brining and spontaneous fermentation with lactic acid bacteria more than doubled extraction of polyphenolics and antioxidants from the rosemary leaves. The results show that lactic acid fermentation enhances antioxidant activity in extracts by increasing the total phenolic content but does not increase extraction of phytosterols. Increased extraction of phenolic oxidants during fermentation assisted extraction, results from the in situ generated natural eutectic solvent from the plant sample. ATR-FTIR spectra from the bioactive bands suggests that this increased antioxidant activity is associated with increased extraction of rosmarinic acid, depolymerised lignin, abietane diterpenoids and 15-hydroxy-7-oxodehydroabietic acid. |
| first_indexed | 2024-03-10T06:53:55Z |
| format | Article |
| id | doaj.art-48b653c03d27472eb70cc56aeee826fc |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:53:55Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-48b653c03d27472eb70cc56aeee826fc2023-11-22T16:36:47ZengMDPI AGMolecules1420-30492021-10-012619606410.3390/molecules26196064HPTLC and ATR/FTIR Characterization of Antioxidants in Different Rosemary ExtractsSnezana Agatonovic-Kustrin0Ksenia S. Balyklova1Vladimir Gegechkori2David W. Morton3Department of Pharmaceutical and Toxicological Chemistry named after Arzamastsev of the Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartment of Pharmaceutical and Toxicological Chemistry named after Arzamastsev of the Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartment of Pharmaceutical and Toxicological Chemistry named after Arzamastsev of the Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartment of Pharmaceutical and Toxicological Chemistry named after Arzamastsev of the Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaThe effect of spontaneous fermentation by lactic acid bacteria on the extraction yield of bioactive compounds and antioxidant activity from rosemary leaf extracts was investigated using high-performance thin-layer chromatography (HPTLC). Brining and spontaneous fermentation with lactic acid bacteria more than doubled extraction of polyphenolics and antioxidants from the rosemary leaves. The results show that lactic acid fermentation enhances antioxidant activity in extracts by increasing the total phenolic content but does not increase extraction of phytosterols. Increased extraction of phenolic oxidants during fermentation assisted extraction, results from the in situ generated natural eutectic solvent from the plant sample. ATR-FTIR spectra from the bioactive bands suggests that this increased antioxidant activity is associated with increased extraction of rosmarinic acid, depolymerised lignin, abietane diterpenoids and 15-hydroxy-7-oxodehydroabietic acid.https://www.mdpi.com/1420-3049/26/19/6064rosemary antioxidantsHPTLC fingerprintsfermentation assisted extractionfractional freezingabietic acidnatural eutectic solvent |
| spellingShingle | Snezana Agatonovic-Kustrin Ksenia S. Balyklova Vladimir Gegechkori David W. Morton HPTLC and ATR/FTIR Characterization of Antioxidants in Different Rosemary Extracts Molecules rosemary antioxidants HPTLC fingerprints fermentation assisted extraction fractional freezing abietic acid natural eutectic solvent |
| title | HPTLC and ATR/FTIR Characterization of Antioxidants in Different Rosemary Extracts |
| title_full | HPTLC and ATR/FTIR Characterization of Antioxidants in Different Rosemary Extracts |
| title_fullStr | HPTLC and ATR/FTIR Characterization of Antioxidants in Different Rosemary Extracts |
| title_full_unstemmed | HPTLC and ATR/FTIR Characterization of Antioxidants in Different Rosemary Extracts |
| title_short | HPTLC and ATR/FTIR Characterization of Antioxidants in Different Rosemary Extracts |
| title_sort | hptlc and atr ftir characterization of antioxidants in different rosemary extracts |
| topic | rosemary antioxidants HPTLC fingerprints fermentation assisted extraction fractional freezing abietic acid natural eutectic solvent |
| url | https://www.mdpi.com/1420-3049/26/19/6064 |
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