Enkapsulasi Probiotik Lactobacillus sp.Menggunakan Dua Tahap Proses

The Lactobacillus sp. a probiotic microorganism that can’t to survive gastric acidity and the concentration of bile salts in the gastrointestinal tract. Probiotic encapsulation is one of the methods to protect probiotic during processing process, storage, and from acidic solutions in the gastrointes...

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Main Authors: Rintan Fransiska Trimudita, Djaenudin Djaenudin
Format: Article
Language:English
Published: University of Serambi Mekkah 2021-03-01
Series:Jurnal Serambi Engineering
Subjects:
Online Access:https://ojs.serambimekkah.ac.id/jse/article/view/2883
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author Rintan Fransiska Trimudita
Djaenudin Djaenudin
author_facet Rintan Fransiska Trimudita
Djaenudin Djaenudin
author_sort Rintan Fransiska Trimudita
collection DOAJ
description The Lactobacillus sp. a probiotic microorganism that can’t to survive gastric acidity and the concentration of bile salts in the gastrointestinal tract. Probiotic encapsulation is one of the methods to protect probiotic during processing process, storage, and from acidic solutions in the gastrointestinal tract. The research was to know that viability was encrypted with an alginat matrix and we were against testing in simulated stomach acid. In this research, the encapsulation process was conducted by mixing Lactobacillus sp. with Na-Alginate to form a suspension as encapsulation material. The microcapsules formed were coated with chitosan with concentrations i.e., 1.2%; 1.6%; and 2%. Lactobacillus sp. encapsulated chitosan matrix was tested for its viability in gastric fluid simulation (0,2% NaCl pH 1,2 and 3) for 1 minute, 60 minutes and 120 minutes using the TPC method (Total Plate Count). After the viability test, Lactobacillus sp. encapsulated with 2% chitosan could maintain the viability lactobacillus sp with the number of colony was of 7,41 log Cfu/gram in the simulation of gastric acid fluid pH 3 for 120 minutes, and 4,78 log Cfu/gram in the simulation gastric acid fluid  pH 1,2 with duration 120 minutes.
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spelling doaj.art-48b81c1d66f34a1fbc03e0d95731e8e32022-12-22T03:48:38ZengUniversity of Serambi MekkahJurnal Serambi Engineering2528-35612541-19342021-03-016210.32672/jse.v6i2.28832409Enkapsulasi Probiotik Lactobacillus sp.Menggunakan Dua Tahap ProsesRintan Fransiska Trimudita0Djaenudin DjaenudinLoka Penelitian Teknologi Bersih – Lembaga Ilmu Pengetahuan Indonesia (LPTB-LIPI) BandungThe Lactobacillus sp. a probiotic microorganism that can’t to survive gastric acidity and the concentration of bile salts in the gastrointestinal tract. Probiotic encapsulation is one of the methods to protect probiotic during processing process, storage, and from acidic solutions in the gastrointestinal tract. The research was to know that viability was encrypted with an alginat matrix and we were against testing in simulated stomach acid. In this research, the encapsulation process was conducted by mixing Lactobacillus sp. with Na-Alginate to form a suspension as encapsulation material. The microcapsules formed were coated with chitosan with concentrations i.e., 1.2%; 1.6%; and 2%. Lactobacillus sp. encapsulated chitosan matrix was tested for its viability in gastric fluid simulation (0,2% NaCl pH 1,2 and 3) for 1 minute, 60 minutes and 120 minutes using the TPC method (Total Plate Count). After the viability test, Lactobacillus sp. encapsulated with 2% chitosan could maintain the viability lactobacillus sp with the number of colony was of 7,41 log Cfu/gram in the simulation of gastric acid fluid pH 3 for 120 minutes, and 4,78 log Cfu/gram in the simulation gastric acid fluid  pH 1,2 with duration 120 minutes.https://ojs.serambimekkah.ac.id/jse/article/view/2883probiotik, enkapsulasi, lactobacillus sp., na-alginat, kitosan
spellingShingle Rintan Fransiska Trimudita
Djaenudin Djaenudin
Enkapsulasi Probiotik Lactobacillus sp.Menggunakan Dua Tahap Proses
Jurnal Serambi Engineering
probiotik, enkapsulasi, lactobacillus sp., na-alginat, kitosan
title Enkapsulasi Probiotik Lactobacillus sp.Menggunakan Dua Tahap Proses
title_full Enkapsulasi Probiotik Lactobacillus sp.Menggunakan Dua Tahap Proses
title_fullStr Enkapsulasi Probiotik Lactobacillus sp.Menggunakan Dua Tahap Proses
title_full_unstemmed Enkapsulasi Probiotik Lactobacillus sp.Menggunakan Dua Tahap Proses
title_short Enkapsulasi Probiotik Lactobacillus sp.Menggunakan Dua Tahap Proses
title_sort enkapsulasi probiotik lactobacillus sp menggunakan dua tahap proses
topic probiotik, enkapsulasi, lactobacillus sp., na-alginat, kitosan
url https://ojs.serambimekkah.ac.id/jse/article/view/2883
work_keys_str_mv AT rintanfransiskatrimudita enkapsulasiprobiotiklactobacillusspmenggunakanduatahapproses
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