Real time PCR based on Fluorescent Quenching of Mercaptoacetic Acid-Modified CdTe Quantum Dots for Ultrasensitive Specific Detection of Nucleic Acids

Real time PCR based on Fluorescent Quenching of Mercaptoacetic Acid-Modified CdTe Quantum Dots for Ultrasensitive Specific Detection of Nucleic Acids

A ultra-sensitive, highly specific, real-time polymerase chain reaction system based on mercaptoacetic acid-modified CdTe nanocrystals(mQDs) is reported. With the addition of 3 nm mQDs into the PCR reagent, the photoluminescent(PL) intensities of mQDs decreased gradually as the DNA templates and PCR...

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Bibliographic Details
Main Authors: Daxiang Cui, Qing Li, Peng Huang, Kan Wang, Yifei Kong, Hong Zhang, Xiaogang You, Rong He, Hua Song, Jingping Wang, Chenchen Bao, Toru Asahi, Feng Gao, Tetsuya Osaka
Format: Article
Language:English
Published: Tsinghua University Press 2010-03-01
Series:Nano Biomedicine and Engineering
Subjects:
mercaptoacetic acid-modified cdte quantum dot
polymerase chain reaction
sensitivity
photoluminescence
specificity
Online Access:https://www.sciopen.com/article/10.5101/nbe.v2i1.p45-55

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https://www.sciopen.com/article/10.5101/nbe.v2i1.p45-55

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