Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates
Here we demonstrate the results of kinetic modeilng of DNA double-strand breaks induction and repair and phosphorilated histone H2AX ($\gamma$-H2AX) and Rad51 foci formation in primary human fibroblasts exposed to low-LET ionizing radiation (IR). The model describes two major paths of DNA double-str...
| Main Authors: | , |
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| Format: | Article |
| Language: | Russian |
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Institute of Computer Science
2015-02-01
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| Series: | Компьютерные исследования и моделирование |
| Subjects: | |
| Online Access: | http://crm.ics.org.ru/uploads/crmissues/crm_2015_1/15112.pdf |
| _version_ | 1818214610465980416 |
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| author | Ivan Vitalievich Ozerov Andreyan Nikolaevich Osipov |
| author_facet | Ivan Vitalievich Ozerov Andreyan Nikolaevich Osipov |
| author_sort | Ivan Vitalievich Ozerov |
| collection | DOAJ |
| description | Here we demonstrate the results of kinetic modeilng of DNA double-strand breaks induction and repair and phosphorilated histone H2AX ($\gamma$-H2AX) and Rad51 foci formation in primary human fibroblasts exposed to low-LET ionizing radiation (IR). The model describes two major paths of DNA double-strand breaks repair: non-homologous end joining (NHEJ) and homologous recombination (HR) and considers interactions between DNA and several repair proteins (DNA-PKcs, ATM, Ku70/80, XRCC1, XRCC4, Rad51, RPA, etc.) using mass action equations and Michaelis-Menten kinetics. Experimental data on DNA rejoining kinetics and $\gamma$-H2AX and Rad51 foci formation in vicinity of double strand breaks in primary human fibroblasts exposed to low-LET IR with various dose rates and exposure times was utilized for training and statistical validation of the model. |
| first_indexed | 2024-12-12T06:22:55Z |
| format | Article |
| id | doaj.art-48d84822be67470f9e8131c94d4caf83 |
| institution | Directory Open Access Journal |
| issn | 2076-7633 2077-6853 |
| language | Russian |
| last_indexed | 2024-12-12T06:22:55Z |
| publishDate | 2015-02-01 |
| publisher | Institute of Computer Science |
| record_format | Article |
| series | Компьютерные исследования и моделирование |
| spelling | doaj.art-48d84822be67470f9e8131c94d4caf832022-12-22T00:34:52ZrusInstitute of Computer ScienceКомпьютерные исследования и моделирование2076-76332077-68532015-02-017115917610.20537/2076-7633-2015-7-1-159-1762237Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose ratesIvan Vitalievich OzerovAndreyan Nikolaevich OsipovHere we demonstrate the results of kinetic modeilng of DNA double-strand breaks induction and repair and phosphorilated histone H2AX ($\gamma$-H2AX) and Rad51 foci formation in primary human fibroblasts exposed to low-LET ionizing radiation (IR). The model describes two major paths of DNA double-strand breaks repair: non-homologous end joining (NHEJ) and homologous recombination (HR) and considers interactions between DNA and several repair proteins (DNA-PKcs, ATM, Ku70/80, XRCC1, XRCC4, Rad51, RPA, etc.) using mass action equations and Michaelis-Menten kinetics. Experimental data on DNA rejoining kinetics and $\gamma$-H2AX and Rad51 foci formation in vicinity of double strand breaks in primary human fibroblasts exposed to low-LET IR with various dose rates and exposure times was utilized for training and statistical validation of the model.http://crm.ics.org.ru/uploads/crmissues/crm_2015_1/15112.pdfDNA double-strand breaksDNA repairkinetic modelinglow-LET ionizing radiationdose rate |
| spellingShingle | Ivan Vitalievich Ozerov Andreyan Nikolaevich Osipov Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates Компьютерные исследования и моделирование DNA double-strand breaks DNA repair kinetic modeling low-LET ionizing radiation dose rate |
| title | Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates |
| title_full | Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates |
| title_fullStr | Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates |
| title_full_unstemmed | Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates |
| title_short | Kinetic model of DNA double-strand break repair in primary human fibroblasts exposed to low-LET irradiation with various dose rates |
| title_sort | kinetic model of dna double strand break repair in primary human fibroblasts exposed to low let irradiation with various dose rates |
| topic | DNA double-strand breaks DNA repair kinetic modeling low-LET ionizing radiation dose rate |
| url | http://crm.ics.org.ru/uploads/crmissues/crm_2015_1/15112.pdf |
| work_keys_str_mv | AT ivanvitalievichozerov kineticmodelofdnadoublestrandbreakrepairinprimaryhumanfibroblastsexposedtolowletirradiationwithvariousdoserates AT andreyannikolaevichosipov kineticmodelofdnadoublestrandbreakrepairinprimaryhumanfibroblastsexposedtolowletirradiationwithvariousdoserates |