Analytical and functional profiles of paralytic shellfish toxins extracted from Semele proficua and Senilia senilis from Angola
Paralytic shellfish poisoning is a foodborne illness that typically derive from the consumption of shellfish contaminated with saxitoxin-group of toxins produced by dinoflagellates of the genus Gymnodinium, Alexandrium and Pyrodinium. N-sulfocarbamoyl, carbamate and dicarbamoyl are the most abundant...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-02-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024013690 |
| _version_ | 1797304471727898624 |
|---|---|
| author | Sandra Raposo-García Ana M. Botana Verónica Rey Celia Costas Luis Rodríguez-Santos M. Carmen Louzao Carmen Vale Luis M. Botana |
| author_facet | Sandra Raposo-García Ana M. Botana Verónica Rey Celia Costas Luis Rodríguez-Santos M. Carmen Louzao Carmen Vale Luis M. Botana |
| author_sort | Sandra Raposo-García |
| collection | DOAJ |
| description | Paralytic shellfish poisoning is a foodborne illness that typically derive from the consumption of shellfish contaminated with saxitoxin-group of toxins produced by dinoflagellates of the genus Gymnodinium, Alexandrium and Pyrodinium. N-sulfocarbamoyl, carbamate and dicarbamoyl are the most abundant. In 2007 and 2008 some episodes of PSP occurred in Angola where there is not monitoring program for shellfish contamination with marine biotoxins. Therefore, ten samples extracted from Semele proficua from Luanda Bay and Senilia senilis from Mussulo Bay, were analyzed by HPLC finding saxitoxin, decarbamoylsaxitoxin and other three compounds that have an unusual profile different to the known hydrophilic PSP toxins were found in different amounts and combinations. These new compounds were not autofluorescent, and they presented much stronger response after peroxide oxidation than after periodate oxidation. The compounds appear as peaks eluted at 2.5 and 5.6 min after periodate oxidation and 8.2 min after peroxide oxidation. Electrophysiological studies revealed that none of the three unknown compounds had effect at cellular level by decreasing the maximum peak inward sodium currents by blocking voltage-gated sodium channels. Thus, not contributing to PSP intoxication. The presence in all samples of saxitoxin-group compounds poses a risk to human health and remarks the need to further explore the presence of new compounds that contaminate seafood, investigating their activity and developing monitoring programs. |
| first_indexed | 2024-03-08T00:09:59Z |
| format | Article |
| id | doaj.art-48da08e2e9b54b4ebe56b1fd524b2d58 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T00:09:59Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-48da08e2e9b54b4ebe56b1fd524b2d582024-02-17T06:40:42ZengElsevierHeliyon2405-84402024-02-01103e25338Analytical and functional profiles of paralytic shellfish toxins extracted from Semele proficua and Senilia senilis from AngolaSandra Raposo-García0Ana M. Botana1Verónica Rey2Celia Costas3Luis Rodríguez-Santos4M. Carmen Louzao5Carmen Vale6Luis M. Botana7Departamento de Farmacologia, IDIS, Universidad de Santiago de Compostela, Campus Universitario, 27002, Lugo, SpainDepartamento de Química Analítica, Universidad de Santiago de Compostela, Campus Universitario, 27002, Lugo, Spain; Corresponding author.Departamento de Química Analítica, Universidad de Santiago de Compostela, Campus Universitario, 27002, Lugo, SpainDepartamento de Farmacologia, IDIS, Universidad de Santiago de Compostela, Campus Universitario, 27002, Lugo, SpainDepartamento de Farmacologia, IDIS, Universidad de Santiago de Compostela, Campus Universitario, 27002, Lugo, SpainDepartamento de Farmacologia, IDIS, Universidad de Santiago de Compostela, Campus Universitario, 27002, Lugo, SpainDepartamento de Farmacologia, IDIS, Universidad de Santiago de Compostela, Campus Universitario, 27002, Lugo, Spain; Corresponding author.Departamento de Farmacologia, IDIS, Universidad de Santiago de Compostela, Campus Universitario, 27002, Lugo, SpainParalytic shellfish poisoning is a foodborne illness that typically derive from the consumption of shellfish contaminated with saxitoxin-group of toxins produced by dinoflagellates of the genus Gymnodinium, Alexandrium and Pyrodinium. N-sulfocarbamoyl, carbamate and dicarbamoyl are the most abundant. In 2007 and 2008 some episodes of PSP occurred in Angola where there is not monitoring program for shellfish contamination with marine biotoxins. Therefore, ten samples extracted from Semele proficua from Luanda Bay and Senilia senilis from Mussulo Bay, were analyzed by HPLC finding saxitoxin, decarbamoylsaxitoxin and other three compounds that have an unusual profile different to the known hydrophilic PSP toxins were found in different amounts and combinations. These new compounds were not autofluorescent, and they presented much stronger response after peroxide oxidation than after periodate oxidation. The compounds appear as peaks eluted at 2.5 and 5.6 min after periodate oxidation and 8.2 min after peroxide oxidation. Electrophysiological studies revealed that none of the three unknown compounds had effect at cellular level by decreasing the maximum peak inward sodium currents by blocking voltage-gated sodium channels. Thus, not contributing to PSP intoxication. The presence in all samples of saxitoxin-group compounds poses a risk to human health and remarks the need to further explore the presence of new compounds that contaminate seafood, investigating their activity and developing monitoring programs.http://www.sciencedirect.com/science/article/pii/S2405844024013690SaxitoxinAlgal bloomsAlgal toxinsCerebellar neuronSodium currentHPLC |
| spellingShingle | Sandra Raposo-García Ana M. Botana Verónica Rey Celia Costas Luis Rodríguez-Santos M. Carmen Louzao Carmen Vale Luis M. Botana Analytical and functional profiles of paralytic shellfish toxins extracted from Semele proficua and Senilia senilis from Angola Heliyon Saxitoxin Algal blooms Algal toxins Cerebellar neuron Sodium current HPLC |
| title | Analytical and functional profiles of paralytic shellfish toxins extracted from Semele proficua and Senilia senilis from Angola |
| title_full | Analytical and functional profiles of paralytic shellfish toxins extracted from Semele proficua and Senilia senilis from Angola |
| title_fullStr | Analytical and functional profiles of paralytic shellfish toxins extracted from Semele proficua and Senilia senilis from Angola |
| title_full_unstemmed | Analytical and functional profiles of paralytic shellfish toxins extracted from Semele proficua and Senilia senilis from Angola |
| title_short | Analytical and functional profiles of paralytic shellfish toxins extracted from Semele proficua and Senilia senilis from Angola |
| title_sort | analytical and functional profiles of paralytic shellfish toxins extracted from semele proficua and senilia senilis from angola |
| topic | Saxitoxin Algal blooms Algal toxins Cerebellar neuron Sodium current HPLC |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024013690 |
| work_keys_str_mv | AT sandraraposogarcia analyticalandfunctionalprofilesofparalyticshellfishtoxinsextractedfromsemeleproficuaandseniliasenilisfromangola AT anambotana analyticalandfunctionalprofilesofparalyticshellfishtoxinsextractedfromsemeleproficuaandseniliasenilisfromangola AT veronicarey analyticalandfunctionalprofilesofparalyticshellfishtoxinsextractedfromsemeleproficuaandseniliasenilisfromangola AT celiacostas analyticalandfunctionalprofilesofparalyticshellfishtoxinsextractedfromsemeleproficuaandseniliasenilisfromangola AT luisrodriguezsantos analyticalandfunctionalprofilesofparalyticshellfishtoxinsextractedfromsemeleproficuaandseniliasenilisfromangola AT mcarmenlouzao analyticalandfunctionalprofilesofparalyticshellfishtoxinsextractedfromsemeleproficuaandseniliasenilisfromangola AT carmenvale analyticalandfunctionalprofilesofparalyticshellfishtoxinsextractedfromsemeleproficuaandseniliasenilisfromangola AT luismbotana analyticalandfunctionalprofilesofparalyticshellfishtoxinsextractedfromsemeleproficuaandseniliasenilisfromangola |