Protocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells

Protocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells

Summary: Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells...

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Bibliographic Details
Main Authors: Serife Ayaz-Guner, Mustafa Burak Acar, Dudu Boyvat, Huseyin Guner, Habibe Bozalan, Melis Güzel, Selin Kübra Yıldır, Nilay Altınsoy, Fatma Fındık, Musa Karakükçü, Servet Özcan
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell Isolation
Protein Biochemistry
Proteomics
Mass Spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007432

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http://www.sciencedirect.com/science/article/pii/S2666166722007432

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