Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Abstract Background Most recombinant Komagataella phaffii (Pichia pastoris) strains for protein production are generated by genomic integration of expression cassettes. The clonal variability in gene copy numbers, integration loci and consequently product titers limit the aptitude for high throughpu...

Full description

Bibliographic Details
Main Authors: Florian Weiss, Guillermo Requena-Moreno, Carsten Pichler, Francisco Valero, Anton Glieder, Xavier Garcia-Ortega
Format: Article
Language:English
Published: BMC 2024-04-01
Series:Microbial Cell Factories
Subjects:
Komagataella phaffii
Pichia pastoris
Episomal ARS plasmid
Lipase CalB
Carbon source marker
GUT1
Online Access:https://doi.org/10.1186/s12934-024-02368-3

Internet

https://doi.org/10.1186/s12934-024-02368-3

Similar Items