Persistence of a Stx-Encoding Bacteriophage in Minced Meat Investigated by Application of an Improved DNA Extraction Method and Digital Droplet PCR

Persistence of a Stx-Encoding Bacteriophage in Minced Meat Investigated by Application of an Improved DNA Extraction Method and Digital Droplet PCR

Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens with Shiga toxins as the main virulence factor. Shiga toxins are encoded on Shiga toxin-encoding bacteriophages (Stx phages). Stx phages may exist as free bacteriophages in the environment or in foods or as prophages in...

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Bibliographic Details
Main Authors: B. Spilsberg, C. Sekse, Anne M. Urdahl, Live L. Nesse, Gro S. Johannessen
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-01-01
Series:Frontiers in Microbiology
Subjects:
stx-phages
STEC
persistence
DNA extraction
digital droplet PCR
minced meat
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2020.581575/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2020.581575/full

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