Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium
Summary: In contrast to mammals, the avian cochlea, specifically the basilar papilla, can regenerate sensory hair cells, which involves fate conversion of supporting cells to hair cells. To determine the mechanisms for converting supporting cells to hair cells, we used single-cell RNA sequencing dur...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223001232 |
| _version_ | 1828012365685194752 |
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| author | Mami Matsunaga Ryosuke Yamamoto Tomoko Kita Hiroe Ohnishi Norio Yamamoto Takayuki Okano Koichi Omori Takayuki Nakagawa |
| author_facet | Mami Matsunaga Ryosuke Yamamoto Tomoko Kita Hiroe Ohnishi Norio Yamamoto Takayuki Okano Koichi Omori Takayuki Nakagawa |
| author_sort | Mami Matsunaga |
| collection | DOAJ |
| description | Summary: In contrast to mammals, the avian cochlea, specifically the basilar papilla, can regenerate sensory hair cells, which involves fate conversion of supporting cells to hair cells. To determine the mechanisms for converting supporting cells to hair cells, we used single-cell RNA sequencing during hair cell regeneration in explant cultures of chick basilar papillae. We identified dynamic changes in the gene expression of supporting cells, and the pseudotime trajectory analysis demonstrated the stepwise fate conversion from supporting cells to hair cells. Initially, supporting cell identity was erased and transition to the precursor state occurred. A subsequent gain in hair cell identity progressed together with downregulation of precursor-state genes. Transforming growth factor β receptor 1-mediated signaling was involved in induction of the initial step, and its inhibition resulted in suppression of hair cell regeneration. Our data provide new insights for understanding fate conversion from supporting cells to hair cells in avian basilar papillae. |
| first_indexed | 2024-04-10T09:30:49Z |
| format | Article |
| id | doaj.art-4914fc989d074300b10dfe74c6722542 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T09:30:49Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-4914fc989d074300b10dfe74c67225422023-02-19T04:27:04ZengElsevieriScience2589-00422023-02-01262106046Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epitheliumMami Matsunaga0Ryosuke Yamamoto1Tomoko Kita2Hiroe Ohnishi3Norio Yamamoto4Takayuki Okano5Koichi Omori6Takayuki Nakagawa7Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanDepartment of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanDepartment of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanDepartment of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanDepartment of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanDepartment of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanDepartment of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanDepartment of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan; Corresponding authorSummary: In contrast to mammals, the avian cochlea, specifically the basilar papilla, can regenerate sensory hair cells, which involves fate conversion of supporting cells to hair cells. To determine the mechanisms for converting supporting cells to hair cells, we used single-cell RNA sequencing during hair cell regeneration in explant cultures of chick basilar papillae. We identified dynamic changes in the gene expression of supporting cells, and the pseudotime trajectory analysis demonstrated the stepwise fate conversion from supporting cells to hair cells. Initially, supporting cell identity was erased and transition to the precursor state occurred. A subsequent gain in hair cell identity progressed together with downregulation of precursor-state genes. Transforming growth factor β receptor 1-mediated signaling was involved in induction of the initial step, and its inhibition resulted in suppression of hair cell regeneration. Our data provide new insights for understanding fate conversion from supporting cells to hair cells in avian basilar papillae.http://www.sciencedirect.com/science/article/pii/S2589004223001232Cell biologySensory neuroscienceTranscriptomics |
| spellingShingle | Mami Matsunaga Ryosuke Yamamoto Tomoko Kita Hiroe Ohnishi Norio Yamamoto Takayuki Okano Koichi Omori Takayuki Nakagawa Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium iScience Cell biology Sensory neuroscience Transcriptomics |
| title | Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium |
| title_full | Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium |
| title_fullStr | Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium |
| title_full_unstemmed | Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium |
| title_short | Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium |
| title_sort | stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium |
| topic | Cell biology Sensory neuroscience Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223001232 |
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