SOLID STATE FERMENTATION AND CHARACTERIZATION OF PARTIALLY PURIFIED THERMOSTABLE MANNANASE FROM Bacillus sp. MG-33
Bacillus sp. MG-33 was isolated from the desert of Rajasthan (India). The organism produced 500 and 200 Ug-1 of thermostable mannanase (after 96h) in solid state fermentation (SSF) of wheat bran and wheat straw rich-soda pulp at the moisture ratio of 1:1.5 and 1:3 at 30ºC, respectively. Two-step par...
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| Language: | English |
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North Carolina State University
2010-06-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_05_3_1689_Meenakshi_SBH_SSF_Char_Mannanase/668 |
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| author | Gursharan Singh Meenakshi Aditya Bhalla Gurinder Singh Hoondal |
| author_facet | Gursharan Singh Meenakshi Aditya Bhalla Gurinder Singh Hoondal |
| author_sort | Gursharan Singh |
| collection | DOAJ |
| description | Bacillus sp. MG-33 was isolated from the desert of Rajasthan (India). The organism produced 500 and 200 Ug-1 of thermostable mannanase (after 96h) in solid state fermentation (SSF) of wheat bran and wheat straw rich-soda pulp at the moisture ratio of 1:1.5 and 1:3 at 30ºC, respectively. Two-step partially purified mannanase was optimally active at 65ºC and was 100% thermostable at 55 to 60ºC for 2h and also retained more than 50% residual activity at 65ºC for 2h. A pH of 6.5 was optimum for enzyme activity and 100% stability up to 4h at this pH. Mannanase activity was slightly enhanced by Ca2+, Fe3+, and Mg2+, while 100% activity was retained in the presence of Ba3+, Li+, and NiCl2 at 1.0-10mM. 1M NaCl and urea did not reduce the enzyme activity. The Km and Vmax of mannanase were 0.2mgml-1 and 60Umg-1ml-1, respectively. Hydrolysis of locust bean was rapid and linear between 5 and 20 min, and ~300µgml-1 mannose was obtained after 20 min of catalytic reaction by enzyme at 65ºC. TLC was used to confirm the mannose as an end product after hydrolysis of locust bean gum. |
| first_indexed | 2024-12-16T17:54:56Z |
| format | Article |
| id | doaj.art-492674cfd2a34ed2ab5fc72df6d95a1b |
| institution | Directory Open Access Journal |
| issn | 1930-2126 |
| language | English |
| last_indexed | 2024-12-16T17:54:56Z |
| publishDate | 2010-06-01 |
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| record_format | Article |
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| spelling | doaj.art-492674cfd2a34ed2ab5fc72df6d95a1b2022-12-21T22:22:12ZengNorth Carolina State UniversityBioResources1930-21262010-06-015316891701SOLID STATE FERMENTATION AND CHARACTERIZATION OF PARTIALLY PURIFIED THERMOSTABLE MANNANASE FROM Bacillus sp. MG-33Gursharan SinghMeenakshiAditya BhallaGurinder Singh HoondalBacillus sp. MG-33 was isolated from the desert of Rajasthan (India). The organism produced 500 and 200 Ug-1 of thermostable mannanase (after 96h) in solid state fermentation (SSF) of wheat bran and wheat straw rich-soda pulp at the moisture ratio of 1:1.5 and 1:3 at 30ºC, respectively. Two-step partially purified mannanase was optimally active at 65ºC and was 100% thermostable at 55 to 60ºC for 2h and also retained more than 50% residual activity at 65ºC for 2h. A pH of 6.5 was optimum for enzyme activity and 100% stability up to 4h at this pH. Mannanase activity was slightly enhanced by Ca2+, Fe3+, and Mg2+, while 100% activity was retained in the presence of Ba3+, Li+, and NiCl2 at 1.0-10mM. 1M NaCl and urea did not reduce the enzyme activity. The Km and Vmax of mannanase were 0.2mgml-1 and 60Umg-1ml-1, respectively. Hydrolysis of locust bean was rapid and linear between 5 and 20 min, and ~300µgml-1 mannose was obtained after 20 min of catalytic reaction by enzyme at 65ºC. TLC was used to confirm the mannose as an end product after hydrolysis of locust bean gum.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_05_3_1689_Meenakshi_SBH_SSF_Char_Mannanase/668Bacillus sp. MG-33MannanaseSolid state fermentationThermophilic |
| spellingShingle | Gursharan Singh Meenakshi Aditya Bhalla Gurinder Singh Hoondal SOLID STATE FERMENTATION AND CHARACTERIZATION OF PARTIALLY PURIFIED THERMOSTABLE MANNANASE FROM Bacillus sp. MG-33 BioResources Bacillus sp. MG-33 Mannanase Solid state fermentation Thermophilic |
| title | SOLID STATE FERMENTATION AND CHARACTERIZATION OF PARTIALLY PURIFIED THERMOSTABLE MANNANASE FROM Bacillus sp. MG-33 |
| title_full | SOLID STATE FERMENTATION AND CHARACTERIZATION OF PARTIALLY PURIFIED THERMOSTABLE MANNANASE FROM Bacillus sp. MG-33 |
| title_fullStr | SOLID STATE FERMENTATION AND CHARACTERIZATION OF PARTIALLY PURIFIED THERMOSTABLE MANNANASE FROM Bacillus sp. MG-33 |
| title_full_unstemmed | SOLID STATE FERMENTATION AND CHARACTERIZATION OF PARTIALLY PURIFIED THERMOSTABLE MANNANASE FROM Bacillus sp. MG-33 |
| title_short | SOLID STATE FERMENTATION AND CHARACTERIZATION OF PARTIALLY PURIFIED THERMOSTABLE MANNANASE FROM Bacillus sp. MG-33 |
| title_sort | solid state fermentation and characterization of partially purified thermostable mannanase from bacillus sp mg 33 |
| topic | Bacillus sp. MG-33 Mannanase Solid state fermentation Thermophilic |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_05_3_1689_Meenakshi_SBH_SSF_Char_Mannanase/668 |
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