Protocol for the rapid intravenous in ovo injection of developing amniote embryos

Summary: We present a technique for precise drug delivery into the vascular system of developing amniote embryos via injection into chorioallantoic veins underlying the eggshell membrane. We describe steps for incubating and candling eggs, removing the shell to expose underlying veins, and precise i...

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Bibliographic Details
Main Authors: Rory L. Cooper, Gabriel Santos-Durán, Michel C. Milinkovitch
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002915