Protocol for the rapid intravenous in ovo injection of developing amniote embryos
Summary: We present a technique for precise drug delivery into the vascular system of developing amniote embryos via injection into chorioallantoic veins underlying the eggshell membrane. We describe steps for incubating and candling eggs, removing the shell to expose underlying veins, and precise i...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723002915 |
| _version_ | 1797823075136307200 |
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| author | Rory L. Cooper Gabriel Santos-Durán Michel C. Milinkovitch |
| author_facet | Rory L. Cooper Gabriel Santos-Durán Michel C. Milinkovitch |
| author_sort | Rory L. Cooper |
| collection | DOAJ |
| description | Summary: We present a technique for precise drug delivery into the vascular system of developing amniote embryos via injection into chorioallantoic veins underlying the eggshell membrane. We describe steps for incubating and candling eggs, removing the shell to expose underlying veins, and precise intravenous injection. In addition to chicken embryos, this protocol is applicable to other amniote species that lay hard-shell eggs, including crocodiles and tortoises. This technique is rapid, is reproducible, is of low cost, and will provide an important resource for developmental biologists.For complete details on the use and execution of this protocol, please refer to Cooper & Milinkovitch.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-13T10:18:39Z |
| format | Article |
| id | doaj.art-49838322c9114b54afd606069d961dcf |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-13T10:18:39Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-49838322c9114b54afd606069d961dcf2023-05-21T04:35:57ZengElsevierSTAR Protocols2666-16672023-06-0142102324Protocol for the rapid intravenous in ovo injection of developing amniote embryosRory L. Cooper0Gabriel Santos-Durán1Michel C. Milinkovitch2Laboratory of Artificial & Natural Evolution (LANE), Department of Genetics and Evolution, University of Geneva, 1211 Geneva, SwitzerlandLaboratory of Artificial & Natural Evolution (LANE), Department of Genetics and Evolution, University of Geneva, 1211 Geneva, SwitzerlandLaboratory of Artificial & Natural Evolution (LANE), Department of Genetics and Evolution, University of Geneva, 1211 Geneva, Switzerland; SIB Swiss Institute of Bioinformatics, Geneva, Switzerland; Corresponding authorSummary: We present a technique for precise drug delivery into the vascular system of developing amniote embryos via injection into chorioallantoic veins underlying the eggshell membrane. We describe steps for incubating and candling eggs, removing the shell to expose underlying veins, and precise intravenous injection. In addition to chicken embryos, this protocol is applicable to other amniote species that lay hard-shell eggs, including crocodiles and tortoises. This technique is rapid, is reproducible, is of low cost, and will provide an important resource for developmental biologists.For complete details on the use and execution of this protocol, please refer to Cooper & Milinkovitch.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723002915Developmental BiologyModel OrganismsEvolutionary Biology |
| spellingShingle | Rory L. Cooper Gabriel Santos-Durán Michel C. Milinkovitch Protocol for the rapid intravenous in ovo injection of developing amniote embryos STAR Protocols Developmental Biology Model Organisms Evolutionary Biology |
| title | Protocol for the rapid intravenous in ovo injection of developing amniote embryos |
| title_full | Protocol for the rapid intravenous in ovo injection of developing amniote embryos |
| title_fullStr | Protocol for the rapid intravenous in ovo injection of developing amniote embryos |
| title_full_unstemmed | Protocol for the rapid intravenous in ovo injection of developing amniote embryos |
| title_short | Protocol for the rapid intravenous in ovo injection of developing amniote embryos |
| title_sort | protocol for the rapid intravenous in ovo injection of developing amniote embryos |
| topic | Developmental Biology Model Organisms Evolutionary Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723002915 |
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