Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids
Abstract Organ-on-a-chip systems combine microfluidics, cell biology, and tissue engineering to culture 3D organ-specific in vitro models that recapitulate the biology and physiology of their in vivo counterparts. Here, we have developed a multiplex platform that automates the culture of individual...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-20096-9 |
| _version_ | 1811186873704382464 |
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| author | Spencer T. Seiler Gary L. Mantalas John Selberg Sergio Cordero Sebastian Torres-Montoya Pierre V. Baudin Victoria T. Ly Finn Amend Liam Tran Ryan N. Hoffman Marco Rolandi Richard E. Green David Haussler Sofie R. Salama Mircea Teodorescu |
| author_facet | Spencer T. Seiler Gary L. Mantalas John Selberg Sergio Cordero Sebastian Torres-Montoya Pierre V. Baudin Victoria T. Ly Finn Amend Liam Tran Ryan N. Hoffman Marco Rolandi Richard E. Green David Haussler Sofie R. Salama Mircea Teodorescu |
| author_sort | Spencer T. Seiler |
| collection | DOAJ |
| description | Abstract Organ-on-a-chip systems combine microfluidics, cell biology, and tissue engineering to culture 3D organ-specific in vitro models that recapitulate the biology and physiology of their in vivo counterparts. Here, we have developed a multiplex platform that automates the culture of individual organoids in isolated microenvironments at user-defined media flow rates. Programmable workflows allow the use of multiple reagent reservoirs that may be applied to direct differentiation, study temporal variables, and grow cultures long term. Novel techniques in polydimethylsiloxane (PDMS) chip fabrication are described here that enable features on the upper and lower planes of a single PDMS substrate. RNA sequencing (RNA-seq) analysis of automated cerebral cortex organoid cultures shows benefits in reducing glycolytic and endoplasmic reticulum stress compared to conventional in vitro cell cultures. |
| first_indexed | 2024-04-11T13:53:35Z |
| format | Article |
| id | doaj.art-49bad5dca74f4ddbb438348058868746 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T13:53:35Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-49bad5dca74f4ddbb4383480588687462022-12-22T04:20:27ZengNature PortfolioScientific Reports2045-23222022-11-0112111210.1038/s41598-022-20096-9Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoidsSpencer T. Seiler0Gary L. Mantalas1John Selberg2Sergio Cordero3Sebastian Torres-Montoya4Pierre V. Baudin5Victoria T. Ly6Finn Amend7Liam Tran8Ryan N. Hoffman9Marco Rolandi10Richard E. Green11David Haussler12Sofie R. Salama13Mircea Teodorescu14UC Santa Cruz Genomics Institute, University of California Santa CruzUC Santa Cruz Genomics Institute, University of California Santa CruzDepartment of Electrical and Computer Engineering, University of California Santa CruzDepartment of Electrical and Computer Engineering, University of California Santa CruzUC Santa Cruz Genomics Institute, University of California Santa CruzUC Santa Cruz Genomics Institute, University of California Santa CruzUC Santa Cruz Genomics Institute, University of California Santa CruzDepartment of Electrical and Computer Engineering, University of California Santa CruzUC Santa Cruz Genomics Institute, University of California Santa CruzUC Santa Cruz Genomics Institute, University of California Santa CruzUC Santa Cruz Genomics Institute, University of California Santa CruzUC Santa Cruz Genomics Institute, University of California Santa CruzUC Santa Cruz Genomics Institute, University of California Santa CruzUC Santa Cruz Genomics Institute, University of California Santa CruzUC Santa Cruz Genomics Institute, University of California Santa CruzAbstract Organ-on-a-chip systems combine microfluidics, cell biology, and tissue engineering to culture 3D organ-specific in vitro models that recapitulate the biology and physiology of their in vivo counterparts. Here, we have developed a multiplex platform that automates the culture of individual organoids in isolated microenvironments at user-defined media flow rates. Programmable workflows allow the use of multiple reagent reservoirs that may be applied to direct differentiation, study temporal variables, and grow cultures long term. Novel techniques in polydimethylsiloxane (PDMS) chip fabrication are described here that enable features on the upper and lower planes of a single PDMS substrate. RNA sequencing (RNA-seq) analysis of automated cerebral cortex organoid cultures shows benefits in reducing glycolytic and endoplasmic reticulum stress compared to conventional in vitro cell cultures.https://doi.org/10.1038/s41598-022-20096-9 |
| spellingShingle | Spencer T. Seiler Gary L. Mantalas John Selberg Sergio Cordero Sebastian Torres-Montoya Pierre V. Baudin Victoria T. Ly Finn Amend Liam Tran Ryan N. Hoffman Marco Rolandi Richard E. Green David Haussler Sofie R. Salama Mircea Teodorescu Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids Scientific Reports |
| title | Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids |
| title_full | Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids |
| title_fullStr | Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids |
| title_full_unstemmed | Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids |
| title_short | Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids |
| title_sort | modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids |
| url | https://doi.org/10.1038/s41598-022-20096-9 |
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