Butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting HMGB1
Butyrate is widely accepted as a proliferation inhibitor in colon cancer but less thoroughly characterized in the colonic epithelium of objects with type 2 diabetes mellitus. The present study investigated the regulatory effect of butyrate on proliferation, the related molecule high-mobility group b...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-04-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861318301634 |
| _version_ | 1819073004272877568 |
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| author | Si-Yi Wang Jie-Yao Li Ji-Hao Xu Zhong-Sheng Xia Di Cheng Wa Zhong Yu Lai Tao Yu Qi-Kui Chen |
| author_facet | Si-Yi Wang Jie-Yao Li Ji-Hao Xu Zhong-Sheng Xia Di Cheng Wa Zhong Yu Lai Tao Yu Qi-Kui Chen |
| author_sort | Si-Yi Wang |
| collection | DOAJ |
| description | Butyrate is widely accepted as a proliferation inhibitor in colon cancer but less thoroughly characterized in the colonic epithelium of objects with type 2 diabetes mellitus. The present study investigated the regulatory effect of butyrate on proliferation, the related molecule high-mobility group box 1 (HMGB1) and the receptor for advanced glycation end products (RAGE) in the colon of db/db type 2 diabetic model mice and non-cancerous NCM460 colon cells. Proliferation and the expression of HMGB1 and RAGE were increased and could be partially reversed by butyrate treatment in the colon of db/db mice, which were consistent in NCM460 cells under a high glucose state. In NCM460 cells, under the normal glucose state, proliferation increased by overexpression of HMGB1. Under a high glucose state, increased expression of HMGB1 was accompanied with a release from cell nuclei into the cytoplasm and extracellular matrix. Down-regulation of HMGB1 could lower the expression of RAGE and attenuate the abnormally increased proliferation. And overexpression of HMGB1 reversed the suppressing effect of butyrate on abnormally increased proliferation. Conclusively, butyrate suppressed the abnormally increased proliferation in colonic epithelial cells under diabetic state by targeting HMGB1. Keywords: Diabetes, Butyrate, Proliferation, HMGB1, Colonic epithelial cells |
| first_indexed | 2024-12-21T17:46:43Z |
| format | Article |
| id | doaj.art-49c998b3d230439c9f1f622c1dee2397 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-21T17:46:43Z |
| publishDate | 2019-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-49c998b3d230439c9f1f622c1dee23972022-12-21T18:55:28ZengElsevierJournal of Pharmacological Sciences1347-86132019-04-011394266274Butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting HMGB1Si-Yi Wang0Jie-Yao Li1Ji-Hao Xu2Zhong-Sheng Xia3Di Cheng4Wa Zhong5Yu Lai6Tao Yu7Qi-Kui Chen8Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang Xi Road, Guangzhou, Guangdong, 510120, People's Republic of ChinaDepartment of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang Xi Road, Guangzhou, Guangdong, 510120, People's Republic of ChinaDepartment of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang Xi Road, Guangzhou, Guangdong, 510120, People's Republic of ChinaDepartment of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang Xi Road, Guangzhou, Guangdong, 510120, People's Republic of ChinaDepartment of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang Xi Road, Guangzhou, Guangdong, 510120, People's Republic of ChinaDepartment of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang Xi Road, Guangzhou, Guangdong, 510120, People's Republic of ChinaDepartment of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang Xi Road, Guangzhou, Guangdong, 510120, People's Republic of ChinaCorresponding author. Fax: +86 20 81332598.; Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang Xi Road, Guangzhou, Guangdong, 510120, People's Republic of ChinaCorresponding author. Fax: +86 20 81332598.; Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang Xi Road, Guangzhou, Guangdong, 510120, People's Republic of ChinaButyrate is widely accepted as a proliferation inhibitor in colon cancer but less thoroughly characterized in the colonic epithelium of objects with type 2 diabetes mellitus. The present study investigated the regulatory effect of butyrate on proliferation, the related molecule high-mobility group box 1 (HMGB1) and the receptor for advanced glycation end products (RAGE) in the colon of db/db type 2 diabetic model mice and non-cancerous NCM460 colon cells. Proliferation and the expression of HMGB1 and RAGE were increased and could be partially reversed by butyrate treatment in the colon of db/db mice, which were consistent in NCM460 cells under a high glucose state. In NCM460 cells, under the normal glucose state, proliferation increased by overexpression of HMGB1. Under a high glucose state, increased expression of HMGB1 was accompanied with a release from cell nuclei into the cytoplasm and extracellular matrix. Down-regulation of HMGB1 could lower the expression of RAGE and attenuate the abnormally increased proliferation. And overexpression of HMGB1 reversed the suppressing effect of butyrate on abnormally increased proliferation. Conclusively, butyrate suppressed the abnormally increased proliferation in colonic epithelial cells under diabetic state by targeting HMGB1. Keywords: Diabetes, Butyrate, Proliferation, HMGB1, Colonic epithelial cellshttp://www.sciencedirect.com/science/article/pii/S1347861318301634 |
| spellingShingle | Si-Yi Wang Jie-Yao Li Ji-Hao Xu Zhong-Sheng Xia Di Cheng Wa Zhong Yu Lai Tao Yu Qi-Kui Chen Butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting HMGB1 Journal of Pharmacological Sciences |
| title | Butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting HMGB1 |
| title_full | Butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting HMGB1 |
| title_fullStr | Butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting HMGB1 |
| title_full_unstemmed | Butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting HMGB1 |
| title_short | Butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting HMGB1 |
| title_sort | butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting hmgb1 |
| url | http://www.sciencedirect.com/science/article/pii/S1347861318301634 |
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