A Fluorescence-Polarization-Based Lipopolysaccharide–Caspase-4 Interaction Assay for the Development of Inhibitors

A Fluorescence-Polarization-Based Lipopolysaccharide–Caspase-4 Interaction Assay for the Development of Inhibitors

Recognition of intracellular lipopolysaccharide (LPS) by Caspase-4 (Casp-4) is critical for host defense against Gram-negative pathogens. LPS binds to the N-terminal caspase activation and recruitment domain (CARD) of procaspase-4, leading to auto-proteolytic activation followed by pro-inflammatory...

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Bibliographic Details
Main Authors: Jinsu An, So Yeon Kim, Eun Gyeong Yang, Hak Suk Chung
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:Molecules
Subjects:
caspase-4
lipopolysaccharides
caspase activation and recruitment domain (CARD)
non-canonical inflammasome
high-throughput screening
fluorescence polarization
Online Access:https://www.mdpi.com/1420-3049/27/8/2458

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https://www.mdpi.com/1420-3049/27/8/2458

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