Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in <i>Komagataella phaffii</i>

Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in <i>Komagataella phaffii</i>

CRISPR (clustered regularly interspaced short palindromic repeats)-based technologies are powerful, programmable tools for site-directed genome modifications. After successful adaptation and efficient use of CRISPR-Cas9 for genome engineering in methylotrophic yeast <i>Komagataella phaffii<...

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Bibliographic Details
Main Authors: Kirill Smirnov, Florian Weiss, Anna-Maria Hatzl, Lukas Rieder, Kjeld Olesen, Sanne Jensen, Anton Glieder
Format: Article
Language:English
Published: MDPI AG 2024-03-01
Series:Journal of Fungi
Subjects:
MAD7 nuclease
CRISPR-Cas9
<i>Komagataella phaffii</i>
<i>Pichia pastoris</i>
genome editing
efficiency
Online Access:https://www.mdpi.com/2309-608X/10/3/197

Internet

https://www.mdpi.com/2309-608X/10/3/197

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