A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells

A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells

Here, we describe a novel method based on intronic MiMIC insertions described in Nagarkar-Jaiswal et al. (2015) to perform conditional gene inactivation in Drosophila. Mosaic analysis in Drosophila cannot be easily performed in post-mitotic cells. We therefore, therefore, developed Flip-Flop, a flip...

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Bibliographic Details
Main Authors: Sonal Nagarkar-Jaiswal, Sathiya N Manivannan, Zhongyuan Zuo, Hugo J Bellen
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2017-05-01
Series:eLife
Subjects:
MiMIC
FLEx
SNF4Aγ
effete
Trim9
post-mitotic cells
Online Access:https://elifesciences.org/articles/26420

Internet

https://elifesciences.org/articles/26420

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