A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells
Here, we describe a novel method based on intronic MiMIC insertions described in Nagarkar-Jaiswal et al. (2015) to perform conditional gene inactivation in Drosophila. Mosaic analysis in Drosophila cannot be easily performed in post-mitotic cells. We therefore, therefore, developed Flip-Flop, a flip...
Main Authors: | Sonal Nagarkar-Jaiswal, Sathiya N Manivannan, Zhongyuan Zuo, Hugo J Bellen |
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Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2017-05-01
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Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/26420 |
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