Caffeic Acid Modulates Processes Associated with Intestinal Inflammation
Caffeic acid is one of the most abundant hydroxycinnamic acids in fruits, vegetables, and beverages. This phenolic compound reaches relevant concentrations in the colon (up to 126 µM) where it could come into contact with the intestinal cells and exert its anti-inflammatory effects. The aim of this...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/13/2/554 |
| _version_ | 1797412759968677888 |
|---|---|
| author | Danuta Zielińska Henryk Zieliński José Moisés Laparra-Llopis Dorota Szawara-Nowak Joanna Honke Juan Antonio Giménez-Bastida |
| author_facet | Danuta Zielińska Henryk Zieliński José Moisés Laparra-Llopis Dorota Szawara-Nowak Joanna Honke Juan Antonio Giménez-Bastida |
| author_sort | Danuta Zielińska |
| collection | DOAJ |
| description | Caffeic acid is one of the most abundant hydroxycinnamic acids in fruits, vegetables, and beverages. This phenolic compound reaches relevant concentrations in the colon (up to 126 µM) where it could come into contact with the intestinal cells and exert its anti-inflammatory effects. The aim of this investigation was to study the capacity of caffeic acid, at plausible concentrations from an in vivo point of view, to modulate mechanisms related to intestinal inflammation. Consequently, we tested the effects of caffeic acid (50–10 µM) on cyclooxygenase (COX)-2 expression and prostaglandin (PG)E<sub>2</sub>, cytokines, and chemokines (IL-8, monocyte chemoattractant protein-1 -MCP-1-, and IL-6) biosynthesis in IL-1β-treated human myofibroblasts of the colon, CCD-18Co. Furthermore, the capacity of caffeic acid to inhibit the angiotensin-converting enzyme (ACE) activity, to hinder advanced glycation end product (AGE) formation, as well as its antioxidant, reducing, and chelating activity were also investigated. Our results showed that (i) caffeic acid targets COX-2 and its product PGE<sub>2</sub> as well as the biosynthesis of IL-8 in the IL-1β-treated cells and (ii) inhibits AGE formation, which could be related to (iii) the high chelating activity exerted. Low anti-ACE, antioxidant, and reducing capacity of caffeic acid was also observed. These effects of caffeic acid expands our knowledge on anti-inflammatory mechanisms against intestinal inflammation. |
| first_indexed | 2024-03-09T05:06:53Z |
| format | Article |
| id | doaj.art-4a178632bce548b298644cfccf43297f |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T05:06:53Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-4a178632bce548b298644cfccf43297f2023-12-03T12:53:14ZengMDPI AGNutrients2072-66432021-02-0113255410.3390/nu13020554Caffeic Acid Modulates Processes Associated with Intestinal InflammationDanuta Zielińska0Henryk Zieliński1José Moisés Laparra-Llopis2Dorota Szawara-Nowak3Joanna Honke4Juan Antonio Giménez-Bastida5Department of Chemistry, University of Warmia and Mazury, 10-727 Olsztyn, PolandDepartment of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research, Polish Academy of Science, 10-748 Olsztyn, PolandGroup of Molecular Immunonutrition in Cancer, Madrid Institute for Advanced Studies in Food (IMDEA-Food), 28049 Madrid, SpainDepartment of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research, Polish Academy of Science, 10-748 Olsztyn, PolandDepartment of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research, Polish Academy of Science, 10-748 Olsztyn, PolandDepartment of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research, Polish Academy of Science, 10-748 Olsztyn, PolandCaffeic acid is one of the most abundant hydroxycinnamic acids in fruits, vegetables, and beverages. This phenolic compound reaches relevant concentrations in the colon (up to 126 µM) where it could come into contact with the intestinal cells and exert its anti-inflammatory effects. The aim of this investigation was to study the capacity of caffeic acid, at plausible concentrations from an in vivo point of view, to modulate mechanisms related to intestinal inflammation. Consequently, we tested the effects of caffeic acid (50–10 µM) on cyclooxygenase (COX)-2 expression and prostaglandin (PG)E<sub>2</sub>, cytokines, and chemokines (IL-8, monocyte chemoattractant protein-1 -MCP-1-, and IL-6) biosynthesis in IL-1β-treated human myofibroblasts of the colon, CCD-18Co. Furthermore, the capacity of caffeic acid to inhibit the angiotensin-converting enzyme (ACE) activity, to hinder advanced glycation end product (AGE) formation, as well as its antioxidant, reducing, and chelating activity were also investigated. Our results showed that (i) caffeic acid targets COX-2 and its product PGE<sub>2</sub> as well as the biosynthesis of IL-8 in the IL-1β-treated cells and (ii) inhibits AGE formation, which could be related to (iii) the high chelating activity exerted. Low anti-ACE, antioxidant, and reducing capacity of caffeic acid was also observed. These effects of caffeic acid expands our knowledge on anti-inflammatory mechanisms against intestinal inflammation.https://www.mdpi.com/2072-6643/13/2/554inflammationcaffeic acidantiglycativeantioxidantmyofibroblastsACE inhibitory activity |
| spellingShingle | Danuta Zielińska Henryk Zieliński José Moisés Laparra-Llopis Dorota Szawara-Nowak Joanna Honke Juan Antonio Giménez-Bastida Caffeic Acid Modulates Processes Associated with Intestinal Inflammation Nutrients inflammation caffeic acid antiglycative antioxidant myofibroblasts ACE inhibitory activity |
| title | Caffeic Acid Modulates Processes Associated with Intestinal Inflammation |
| title_full | Caffeic Acid Modulates Processes Associated with Intestinal Inflammation |
| title_fullStr | Caffeic Acid Modulates Processes Associated with Intestinal Inflammation |
| title_full_unstemmed | Caffeic Acid Modulates Processes Associated with Intestinal Inflammation |
| title_short | Caffeic Acid Modulates Processes Associated with Intestinal Inflammation |
| title_sort | caffeic acid modulates processes associated with intestinal inflammation |
| topic | inflammation caffeic acid antiglycative antioxidant myofibroblasts ACE inhibitory activity |
| url | https://www.mdpi.com/2072-6643/13/2/554 |
| work_keys_str_mv | AT danutazielinska caffeicacidmodulatesprocessesassociatedwithintestinalinflammation AT henrykzielinski caffeicacidmodulatesprocessesassociatedwithintestinalinflammation AT josemoiseslaparrallopis caffeicacidmodulatesprocessesassociatedwithintestinalinflammation AT dorotaszawaranowak caffeicacidmodulatesprocessesassociatedwithintestinalinflammation AT joannahonke caffeicacidmodulatesprocessesassociatedwithintestinalinflammation AT juanantoniogimenezbastida caffeicacidmodulatesprocessesassociatedwithintestinalinflammation |