Design and Evaluation of a Novel Multiplex Real-Time PCR Melting Curve Assay for the Simultaneous Detection of Nine Sexually Transmitted Disease Pathogens in Genitourinary Secretions

Design and Evaluation of a Novel Multiplex Real-Time PCR Melting Curve Assay for the Simultaneous Detection of Nine Sexually Transmitted Disease Pathogens in Genitourinary Secretions

Background: Sexually transmitted diseases (STD) are a major cause of infertility, long-term disability, ectopic pregnancy, and premature birth. Therefore, the development of fast and low-cost laboratory STD diagnostic screening methods will contribute to reducing STD-induced reproductive tract damag...

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Bibliographic Details
Main Authors: Xiao-Mei Hu, Jiang-Xia Xu, Li-Xia Jiang, Lian-Rui Deng, Zhen-Mei Gu, Xiao-Ying Xie, Hui-Cai Ji, Wei-Hua Wang, Li-Ming Li, Cheng-Nan Tian, Fang-Li Song, Shao Huang, Lei Zheng, Tian-Yu Zhong
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-11-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
multiplex
polymerase chain reaction
sanger sequencing
sexually transmitted diseases
pathogen
Online Access:https://www.frontiersin.org/article/10.3389/fcimb.2019.00382/full

Internet

https://www.frontiersin.org/article/10.3389/fcimb.2019.00382/full

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