A comparison of strategies for generating artificial replicates in RNA-seq experiments

Abstract Due to the overall high costs, technical replicates are usually omitted in RNA-seq experiments, but several methods exist to generate them artificially. Bootstrapping reads from FASTQ-files has recently been used in the context of other NGS analyses and can be used to generate artificial te...

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Bibliographic Details
Main Authors: Babak Saremi, Frederic Gusmag, Ottmar Distl, Frank Schaarschmidt, Julia Metzger, Stefanie Becker, Klaus Jung
Format: Article
Language:English
Published: Nature Portfolio 2022-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-11302-9