Improved methanol tolerance of Rhizomucor miehei lipase based on N‑glycosylation within the α-helix region and its application in biodiesel production

Improved methanol tolerance of Rhizomucor miehei lipase based on N‑glycosylation within the α-helix region and its application in biodiesel production

Abstract Background Liquid lipases are widely used to convert oil into biodiesel. Methanol-resistant lipases with high catalytic activity are the first choice for practical production. Rhizomucor miehei lipase (RML) is a single-chain α/β-type protein that is widely used in biodiesel preparation. Imp...

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Bibliographic Details
Main Authors: Miao Tian, Lingmei Yang, Zhiyuan Wang, Pengmei Lv, Junying Fu, Changlin Miao, Ming Li, Tao Liu, Wen Luo
Format: Article
Language:English
Published: BMC 2021-12-01
Series:Biotechnology for Biofuels
Subjects:
Rhizomucor miehei lipase
α-Helix region
N‑Glycosylation
Methanol tolerance
Biodiesel
Online Access:https://doi.org/10.1186/s13068-021-02087-6

Internet

https://doi.org/10.1186/s13068-021-02087-6

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