Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis

Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis

Core fucosylation of N-linked glycoproteins has been linked to physiological and pathological processes. Here, the authors develop a mass spectrometry-based method that utilizes Endo F3 followed by PNGase F treatment to quantify site-specific glycoprotein core fucosylation in protein mixtures.

Bibliographic Details
Main Authors: Liwei Cao, T. Mamie Lih, Yingwei Hu, Michael Schnaubelt, Shao-Yung Chen, Yangying Zhou, Chuanyu Guo, Mingming Dong, Weiming Yang, Rodrigo Vargas Eguez, Lijun Chen, David J. Clark, Akrit Sodhi, Qing Kay Li, Hui Zhang
Format: Article
Language:English
Published: Nature Portfolio 2022-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-31472-4

Internet

https://doi.org/10.1038/s41467-022-31472-4

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