Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis
Core fucosylation of N-linked glycoproteins has been linked to physiological and pathological processes. Here, the authors develop a mass spectrometry-based method that utilizes Endo F3 followed by PNGase F treatment to quantify site-specific glycoprotein core fucosylation in protein mixtures.
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-31472-4 |
| _version_ | 1811323567832301568 |
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| author | Liwei Cao T. Mamie Lih Yingwei Hu Michael Schnaubelt Shao-Yung Chen Yangying Zhou Chuanyu Guo Mingming Dong Weiming Yang Rodrigo Vargas Eguez Lijun Chen David J. Clark Akrit Sodhi Qing Kay Li Hui Zhang |
| author_facet | Liwei Cao T. Mamie Lih Yingwei Hu Michael Schnaubelt Shao-Yung Chen Yangying Zhou Chuanyu Guo Mingming Dong Weiming Yang Rodrigo Vargas Eguez Lijun Chen David J. Clark Akrit Sodhi Qing Kay Li Hui Zhang |
| author_sort | Liwei Cao |
| collection | DOAJ |
| description | Core fucosylation of N-linked glycoproteins has been linked to physiological and pathological processes. Here, the authors develop a mass spectrometry-based method that utilizes Endo F3 followed by PNGase F treatment to quantify site-specific glycoprotein core fucosylation in protein mixtures. |
| first_indexed | 2024-04-13T13:57:31Z |
| format | Article |
| id | doaj.art-4a550a4b508b415991dff817493ba74d |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-13T13:57:31Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-4a550a4b508b415991dff817493ba74d2022-12-22T02:44:08ZengNature PortfolioNature Communications2041-17232022-07-0113111210.1038/s41467-022-31472-4Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysisLiwei Cao0T. Mamie Lih1Yingwei Hu2Michael Schnaubelt3Shao-Yung Chen4Yangying Zhou5Chuanyu Guo6Mingming Dong7Weiming Yang8Rodrigo Vargas Eguez9Lijun Chen10David J. Clark11Akrit Sodhi12Qing Kay Li13Hui Zhang14Department of Pathology, Johns Hopkins UniversityDepartment of Pathology, Johns Hopkins UniversityDepartment of Pathology, Johns Hopkins UniversityDepartment of Pathology, Johns Hopkins UniversityDepartment of Pathology, Johns Hopkins UniversityDepartment of Pathology, Johns Hopkins UniversityWilmer Eye Institute, Johns Hopkins University School of MedicineDepartment of Pathology, Johns Hopkins UniversityDepartment of Pathology, Johns Hopkins UniversityDepartment of Pathology, Johns Hopkins UniversityDepartment of Pathology, Johns Hopkins UniversityDepartment of Pathology, Johns Hopkins UniversityWilmer Eye Institute, Johns Hopkins University School of MedicineDepartment of Pathology, Johns Hopkins UniversityDepartment of Pathology, Johns Hopkins UniversityCore fucosylation of N-linked glycoproteins has been linked to physiological and pathological processes. Here, the authors develop a mass spectrometry-based method that utilizes Endo F3 followed by PNGase F treatment to quantify site-specific glycoprotein core fucosylation in protein mixtures.https://doi.org/10.1038/s41467-022-31472-4 |
| spellingShingle | Liwei Cao T. Mamie Lih Yingwei Hu Michael Schnaubelt Shao-Yung Chen Yangying Zhou Chuanyu Guo Mingming Dong Weiming Yang Rodrigo Vargas Eguez Lijun Chen David J. Clark Akrit Sodhi Qing Kay Li Hui Zhang Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis Nature Communications |
| title | Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis |
| title_full | Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis |
| title_fullStr | Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis |
| title_full_unstemmed | Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis |
| title_short | Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis |
| title_sort | characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis |
| url | https://doi.org/10.1038/s41467-022-31472-4 |
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