Tryptase activates enteric glial cells followed by affecting neuronal properties possibly via the stimuli-associated mediators
Objectives: Mast cell-derived tryptase causes neuronal elongation/sensitization leading to visceral hypersensitivity. However, effects of tryptase on enteric glial cells (EGCs) and subsequent interaction between EGCs and neurons remain unknown. Methods: We evaluated proteins and mRNA expressions in...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Journal of Pharmacological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861323000063 |
| _version_ | 1797869831386562560 |
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| author | Kazunari Tominaga Yoshiko Fujikawa |
| author_facet | Kazunari Tominaga Yoshiko Fujikawa |
| author_sort | Kazunari Tominaga |
| collection | DOAJ |
| description | Objectives: Mast cell-derived tryptase causes neuronal elongation/sensitization leading to visceral hypersensitivity. However, effects of tryptase on enteric glial cells (EGCs) and subsequent interaction between EGCs and neurons remain unknown. Methods: We evaluated proteins and mRNA expressions in EGC (CRL-2690, ATCC) after tryptase stimulation: nerve growth factor (NGF), netrin-1, and glial cell-derived neurotrophic factor (GDNF). We examined morphological changes in neurons (PC12 cells, CRL-1721.1) by co-incubation with the conditioned medium of EGCs after tryptase stimulation. Results: EGC was activated by tryptase, and proliferated (by 1.8-fold) with cytoplasmic expansion and process elongation. Intercellular connections of EGC were more complexed. Tryptase induced mRNA expression (2.5-fold) and protein expression of NGF. Netrin-1 (3-fold) and GDNF (3-fold) mRNA expressions were increased at 30 min. Increase in netrin-1 continued until 6 h, whereas the latter decreased by 3 h. The conditioned medium of EGC after tryptase stimulation expanded neuronal cytoplasm (round or ramified shapes) and neurite outgrowth with elongation of cytoskeletal filaments in time-dependent and dose-dependent manners. These changes were similar to those after NGF stimulation. Growth cone proteins of neurons were also increased by the conditioned medium. Conclusion: EGC activated by tryptase changes neuronal morphology (process elongation and cytoplasm expansion) possibly via the stimuli-associated mediators. |
| first_indexed | 2024-04-10T00:17:49Z |
| format | Article |
| id | doaj.art-4a815657e03246e38d0238b1c0463589 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-04-10T00:17:49Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-4a815657e03246e38d0238b1c04635892023-03-16T05:03:13ZengElsevierJournal of Pharmacological Sciences1347-86132023-04-011514163170Tryptase activates enteric glial cells followed by affecting neuronal properties possibly via the stimuli-associated mediatorsKazunari Tominaga0Yoshiko Fujikawa1Department of Gastroenterology, JCHO Hoshigaoka Medical Center, 4-8-1, Hoshigaoka, Hirakata, 573-8511, Japan; Corresponding author. Fax: +81 72 840 2266.Department of Internal Medicine, Higashisumiyoshi Morimoto Hospital, 3-2-66, Takaai, Higashi-Sumiyoshi-Ku, Osaka, 546-0014, JapanObjectives: Mast cell-derived tryptase causes neuronal elongation/sensitization leading to visceral hypersensitivity. However, effects of tryptase on enteric glial cells (EGCs) and subsequent interaction between EGCs and neurons remain unknown. Methods: We evaluated proteins and mRNA expressions in EGC (CRL-2690, ATCC) after tryptase stimulation: nerve growth factor (NGF), netrin-1, and glial cell-derived neurotrophic factor (GDNF). We examined morphological changes in neurons (PC12 cells, CRL-1721.1) by co-incubation with the conditioned medium of EGCs after tryptase stimulation. Results: EGC was activated by tryptase, and proliferated (by 1.8-fold) with cytoplasmic expansion and process elongation. Intercellular connections of EGC were more complexed. Tryptase induced mRNA expression (2.5-fold) and protein expression of NGF. Netrin-1 (3-fold) and GDNF (3-fold) mRNA expressions were increased at 30 min. Increase in netrin-1 continued until 6 h, whereas the latter decreased by 3 h. The conditioned medium of EGC after tryptase stimulation expanded neuronal cytoplasm (round or ramified shapes) and neurite outgrowth with elongation of cytoskeletal filaments in time-dependent and dose-dependent manners. These changes were similar to those after NGF stimulation. Growth cone proteins of neurons were also increased by the conditioned medium. Conclusion: EGC activated by tryptase changes neuronal morphology (process elongation and cytoplasm expansion) possibly via the stimuli-associated mediators.http://www.sciencedirect.com/science/article/pii/S1347861323000063Visceral hypersensitivityIrritable bowel syndromeEnteric glial cellNerve growth factorNeuronal elongation factors |
| spellingShingle | Kazunari Tominaga Yoshiko Fujikawa Tryptase activates enteric glial cells followed by affecting neuronal properties possibly via the stimuli-associated mediators Journal of Pharmacological Sciences Visceral hypersensitivity Irritable bowel syndrome Enteric glial cell Nerve growth factor Neuronal elongation factors |
| title | Tryptase activates enteric glial cells followed by affecting neuronal properties possibly via the stimuli-associated mediators |
| title_full | Tryptase activates enteric glial cells followed by affecting neuronal properties possibly via the stimuli-associated mediators |
| title_fullStr | Tryptase activates enteric glial cells followed by affecting neuronal properties possibly via the stimuli-associated mediators |
| title_full_unstemmed | Tryptase activates enteric glial cells followed by affecting neuronal properties possibly via the stimuli-associated mediators |
| title_short | Tryptase activates enteric glial cells followed by affecting neuronal properties possibly via the stimuli-associated mediators |
| title_sort | tryptase activates enteric glial cells followed by affecting neuronal properties possibly via the stimuli associated mediators |
| topic | Visceral hypersensitivity Irritable bowel syndrome Enteric glial cell Nerve growth factor Neuronal elongation factors |
| url | http://www.sciencedirect.com/science/article/pii/S1347861323000063 |
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