Development of a Genus-Specific <i>Brucella</i> Real-Time PCR Assay Targeting the 16S-23S rDNA Internal Transcribed Spacer from Different Specimen Types

The aim of this study was to develop a 16S-23S ribosomal deoxyribonucleic acid internal transcribed spacer (ITS) quantitative polymerase chain reaction (qPCR) assay for the early diagnosis and rapid screening of brucellosis. Blood, milk, and tissue samples were spiked with <i>B. abortus</i&...

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Bibliographic Details
Main Authors: Rejoice Nyarku, Ayesha Hassim, Annelize Jonker, Melvyn Quan
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Veterinary Sciences
Subjects:
Online Access:https://www.mdpi.com/2306-7381/7/4/175