Does pre-incubation in selective-enrichment media improve the detection of diarrheagenic Escherichia coli using the RIDA®GENE PCR?

We aimed to investigate whether a selective pre-PCR enrichment step improves test performance of RIDA®GENE EHEC/EPEC to detect diarrheagenic Escherichia coli from stool samples. Each of the 250 stool samples was analyzed for the presence of stx1/2 and eae both with and without pre-PCR enrichment in...

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Bibliographic Details
Main Authors: Neele J. Froböse, Ioana D. Olaru, Julia Sophie Schneider, Wenlan Zhang, Alexander Mellmann, Franziska Schuler, Tobias Grebe, Frieder Schaumburg
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:International Journal of Medical Microbiology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1438422123000036