Validation of COI metabarcoding primers for terrestrial arthropods

Validation of COI metabarcoding primers for terrestrial arthropods

Metabarcoding can rapidly determine the species composition of bulk samples and thus aids biodiversity and ecosystem assessment. However, it is essential to use primer sets that minimize amplification bias among taxa to maximize species recovery. Despite this fact, the performance of primer sets emp...

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Bibliographic Details
Main Authors: Vasco Elbrecht, Thomas W.A. Braukmann, Natalia V. Ivanova, Sean W.J. Prosser, Mehrdad Hajibabaei, Michael Wright, Evgeny V. Zakharov, Paul D.N. Hebert, Dirk Steinke
Format: Article
Language:English
Published: PeerJ Inc. 2019-10-01
Series:PeerJ
Subjects:
DNA metabarcoding
Primer bias
Degeneracy
Insects
Biodiversity
Online Access:https://peerj.com/articles/7745.pdf

Internet

https://peerj.com/articles/7745.pdf

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