Context-dependent genomic locus effects on antibody production in recombinant Chinese hamster ovary cells generated through random integration

Context-dependent genomic locus effects on antibody production in recombinant Chinese hamster ovary cells generated through random integration

High-yield production of therapeutic protein using Chinese hamster ovary (CHO) cells requires stable cell line development (CLD). CLD typically uses random integration of transgenes; however, this results in clonal variation and subsequent laborious clone screening. Therefore, site-specific integrat...

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Bibliographic Details
Main Authors: Hyun Jee Woo, Jaehoon Kim, Seul Mi Kim, Dongwoo Kim, Jae Yun Moon, Daechan Park, Jae Seong Lee
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Computational and Structural Biotechnology Journal
Subjects:
Chinese hamster ovary (CHO)
Cell line development
Hot spot
Random integration
Online Access:http://www.sciencedirect.com/science/article/pii/S2001037024001016

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http://www.sciencedirect.com/science/article/pii/S2001037024001016

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