Combined TP53 status in tumor-free resection margins and circulating microRNA profiling predicts the risk of locoregional recurrence in head and neck cancer
Abstract Locoregional recurrences represent a frequently unexpected problem in head and neck squamous cell carcinoma (HNSCC). Relapse often (10–30%) occurs in patients with histologically negative resection margins (RMs), probably due to residual tumor cells or hidden pre-cancerous lesions in normal...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | Biomarker Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40364-024-00576-y |
| _version_ | 1797233386924802048 |
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| author | Federica Ganci Matteo Allegretti Carlotta Frascolla Francesca Spinella Francesca Rollo Andrea Sacconi Pascale De Valentina Alina Catalina Palcau Valentina Manciocco Mariavittoria Vescovo Ettore Cotroneo Francesca Blandino Maria Benevolo Renato Covello Paola Muti Sabrina Strano Antonello Vidiri Giulia Fontemaggi Raul Pellini Giovanni Blandino |
| author_facet | Federica Ganci Matteo Allegretti Carlotta Frascolla Francesca Spinella Francesca Rollo Andrea Sacconi Pascale De Valentina Alina Catalina Palcau Valentina Manciocco Mariavittoria Vescovo Ettore Cotroneo Francesca Blandino Maria Benevolo Renato Covello Paola Muti Sabrina Strano Antonello Vidiri Giulia Fontemaggi Raul Pellini Giovanni Blandino |
| author_sort | Federica Ganci |
| collection | DOAJ |
| description | Abstract Locoregional recurrences represent a frequently unexpected problem in head and neck squamous cell carcinoma (HNSCC). Relapse often (10–30%) occurs in patients with histologically negative resection margins (RMs), probably due to residual tumor cells or hidden pre-cancerous lesions in normal mucosa, both missed by histopathological examination. Therefore, definition of a ‘clean’ or tumor-negative RM is controversial, demanding for novel approaches to be accurately explored. Here, we evaluated next generation sequencing (NGS) and digital PCR (dPCR) as tools to profile TP53 mutational status and circulating microRNA expression aiming at scoring the locoregional risk of recurrence by means of molecular analyses. Serial monitoring of these biomarkers allowed identifying patients at high risk, laying the ground for accurate tracking of disease evolution and potential intensification of post-operative treatments. Additionally, our pipeline demonstrated its applicability into the clinical routine, being cost-effective and feasible in terms of patient sampling, holding promise to accurately (re)-stage RMs in the era of precision medicine. |
| first_indexed | 2024-04-24T16:15:21Z |
| format | Article |
| id | doaj.art-4aec13763f344428a3a4e758c3570cc9 |
| institution | Directory Open Access Journal |
| issn | 2050-7771 |
| language | English |
| last_indexed | 2024-04-24T16:15:21Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Biomarker Research |
| spelling | doaj.art-4aec13763f344428a3a4e758c3570cc92024-03-31T11:27:35ZengBMCBiomarker Research2050-77712024-03-011211510.1186/s40364-024-00576-yCombined TP53 status in tumor-free resection margins and circulating microRNA profiling predicts the risk of locoregional recurrence in head and neck cancerFederica Ganci0Matteo Allegretti1Carlotta Frascolla2Francesca Spinella3Francesca Rollo4Andrea Sacconi5Pascale De Valentina6Alina Catalina Palcau7Valentina Manciocco8Mariavittoria Vescovo9Ettore Cotroneo10Francesca Blandino11Maria Benevolo12Renato Covello13Paola Muti14Sabrina Strano15Antonello Vidiri16Giulia Fontemaggi17Raul Pellini18Giovanni Blandino19Translational Oncologic Research Unit, IRCCS Regina Elena National Cancer InstituteTranslational Oncologic Research Unit, IRCCS Regina Elena National Cancer InstituteTranslational Oncologic Research Unit, IRCCS Regina Elena National Cancer InstituteDepartment of Research and Development, Eurofins Genoma GroupPathology, IRCCS Regina Elena National Cancer InstituteSAFU Unit, IRCCS Regina Elena National Cancer InstituteTranslational Oncologic Research Unit, IRCCS Regina Elena National Cancer InstituteTranslational Oncologic Research Unit, IRCCS Regina Elena National Cancer InstituteOtolaryngology-Head and Neck Surgery, IRCCS Regina Elena National Cancer InstitutePathology, IRCCS Regina Elena National Cancer InstituteClinical and Technical Department Management, Eurofins Genoma GroupDepartment of Research and Development, Eurofins Genoma GroupPathology, IRCCS Regina Elena National Cancer InstitutePathology, IRCCS Regina Elena National Cancer InstituteDepartment of Health Research Methods, Evidence, and Impact, Faculty of Health Sciences, McMaster UniversitySAFU Unit, IRCCS Regina Elena National Cancer InstituteRadiology and Diagnostic Imaging, IRCCS Regina Elena National Cancer InstituteTranslational Oncologic Research Unit, IRCCS Regina Elena National Cancer InstituteOtolaryngology-Head and Neck Surgery, IRCCS Regina Elena National Cancer InstituteTranslational Oncologic Research Unit, IRCCS Regina Elena National Cancer InstituteAbstract Locoregional recurrences represent a frequently unexpected problem in head and neck squamous cell carcinoma (HNSCC). Relapse often (10–30%) occurs in patients with histologically negative resection margins (RMs), probably due to residual tumor cells or hidden pre-cancerous lesions in normal mucosa, both missed by histopathological examination. Therefore, definition of a ‘clean’ or tumor-negative RM is controversial, demanding for novel approaches to be accurately explored. Here, we evaluated next generation sequencing (NGS) and digital PCR (dPCR) as tools to profile TP53 mutational status and circulating microRNA expression aiming at scoring the locoregional risk of recurrence by means of molecular analyses. Serial monitoring of these biomarkers allowed identifying patients at high risk, laying the ground for accurate tracking of disease evolution and potential intensification of post-operative treatments. Additionally, our pipeline demonstrated its applicability into the clinical routine, being cost-effective and feasible in terms of patient sampling, holding promise to accurately (re)-stage RMs in the era of precision medicine.https://doi.org/10.1186/s40364-024-00576-yHNSCCResection marginsLocal recurrenceTP53microRNA profilingLiquid biopsy |
| spellingShingle | Federica Ganci Matteo Allegretti Carlotta Frascolla Francesca Spinella Francesca Rollo Andrea Sacconi Pascale De Valentina Alina Catalina Palcau Valentina Manciocco Mariavittoria Vescovo Ettore Cotroneo Francesca Blandino Maria Benevolo Renato Covello Paola Muti Sabrina Strano Antonello Vidiri Giulia Fontemaggi Raul Pellini Giovanni Blandino Combined TP53 status in tumor-free resection margins and circulating microRNA profiling predicts the risk of locoregional recurrence in head and neck cancer Biomarker Research HNSCC Resection margins Local recurrence TP53 microRNA profiling Liquid biopsy |
| title | Combined TP53 status in tumor-free resection margins and circulating microRNA profiling predicts the risk of locoregional recurrence in head and neck cancer |
| title_full | Combined TP53 status in tumor-free resection margins and circulating microRNA profiling predicts the risk of locoregional recurrence in head and neck cancer |
| title_fullStr | Combined TP53 status in tumor-free resection margins and circulating microRNA profiling predicts the risk of locoregional recurrence in head and neck cancer |
| title_full_unstemmed | Combined TP53 status in tumor-free resection margins and circulating microRNA profiling predicts the risk of locoregional recurrence in head and neck cancer |
| title_short | Combined TP53 status in tumor-free resection margins and circulating microRNA profiling predicts the risk of locoregional recurrence in head and neck cancer |
| title_sort | combined tp53 status in tumor free resection margins and circulating microrna profiling predicts the risk of locoregional recurrence in head and neck cancer |
| topic | HNSCC Resection margins Local recurrence TP53 microRNA profiling Liquid biopsy |
| url | https://doi.org/10.1186/s40364-024-00576-y |
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