One-pot making of sequence-restricted DNA dumbbells
A method to build sequence-restricted DNA dumbbells was developed. 5′-exonuclease converts end sequences of DNA targets into sticky ends. Self-looping oligonucleotides with complementary 3′-overhangs are ligated to form dumbbells by DNA polymerase and ligase in a sequence-restricted manner. These re...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-05-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023032425 |