High Reproducibility of ELISPOT Counts from Nine Different Laboratories

High Reproducibility of ELISPOT Counts from Nine Different Laboratories

The primary goal of immune monitoring with ELISPOT is to measure the number of T cells, specific for any antigen, accurately and reproducibly between different laboratories. In ELISPOT assays, antigen-specific T cells secrete cytokines, forming spots of different sizes on a membrane with variable ba...

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Bibliographic Details
Main Authors: Srividya Sundararaman, Alexey Y. Karulin, Tameem Ansari, Nadine BenHamouda, Judith Gottwein, Sreenivas Laxmanan, Steven M. Levine, John T. Loffredo, Stephanie McArdle, Christine Neudoerfl, Diana Roen, Karina Silina, Mackenzie Welch, Paul V. Lehmann
Format: Article
Language:English
Published: MDPI AG 2015-01-01
Series:Cells
Subjects:
ELISPOT
Smart Count™
Log Normal distribution
harmonization
Online Access:http://www.mdpi.com/2073-4409/4/1/21

Internet

http://www.mdpi.com/2073-4409/4/1/21

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