Technical challenges regarding the use of formalin-fixed paraffin embedded (FFPE) tissue specimens for the detection of bacterial alterations in colorectal cancer

Technical challenges regarding the use of formalin-fixed paraffin embedded (FFPE) tissue specimens for the detection of bacterial alterations in colorectal cancer

Abstract Background Formalin-fixed paraffin embedded (FFPE) tissues may provide an exciting resource to study microbial associations in human disease, but the use of these low biomass specimens remains challenging. We aimed to reduce unintentional bacterial interference in molecular analysis of FFPE...

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Bibliographic Details
Main Authors: Suk Yee Lam, Athanasia Ioannou, Prokopis Konstanti, Thijmen Visseren, Michail Doukas, Maikel Petrus Peppelenbosch, Clara Belzer, Gwenny Manel Fuhler
Format: Article
Language:English
Published: BMC 2021-10-01
Series:BMC Microbiology
Subjects:
DNA contamination
Gastrointestinal microbiome
High-throughput nucleotide sequencing
Formalin-fixed paraffin embedded
Low biomass
Colorectal neoplasms
Online Access:https://doi.org/10.1186/s12866-021-02359-z

Internet

https://doi.org/10.1186/s12866-021-02359-z

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