DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR QUANTIFICATION OF PINOLINE AND ITS METABOLITE 6-OH-1,2,3,4-TETRAHYDROCARBOLINE FOR THE PURPOSE OF CYPD26 ACTIVITY DETERMINATION
A sensitive method for quantification of pinoline and its metabolite in human plasma using solid phase extraction and high performance liquid chromatography with mass-spectrometry was developed. Analytical range was 250-1500 pg/ml both for pinoline and for 6-hydroxy-1,2,3,4-tetrahydro-β-carboline. T...
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| Format: | Article |
| Language: | Russian |
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LLC Center of Pharmaceutical Analytics (LLC «CPHA»)
2019-01-01
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| Series: | Разработка и регистрация лекарственных средств |
| Subjects: | |
| Online Access: | https://www.pharmjournal.ru/jour/article/view/240 |
| _version_ | 1826569800901459968 |
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| author | R. K. Abdrashitov A. E. Petukhov V. V. Smirnov |
| author_facet | R. K. Abdrashitov A. E. Petukhov V. V. Smirnov |
| author_sort | R. K. Abdrashitov |
| collection | DOAJ |
| description | A sensitive method for quantification of pinoline and its metabolite in human plasma using solid phase extraction and high performance liquid chromatography with mass-spectrometry was developed. Analytical range was 250-1500 pg/ml both for pinoline and for 6-hydroxy-1,2,3,4-tetrahydro-β-carboline. The developed method may be used to determine the current status of CYP2D6 activity. |
| first_indexed | 2024-04-10T01:41:16Z |
| format | Article |
| id | doaj.art-4ba17a4156d648f9bd42e4a925047470 |
| institution | Directory Open Access Journal |
| issn | 2305-2066 2658-5049 |
| language | Russian |
| last_indexed | 2025-03-14T11:45:06Z |
| publishDate | 2019-01-01 |
| publisher | LLC Center of Pharmaceutical Analytics (LLC «CPHA») |
| record_format | Article |
| series | Разработка и регистрация лекарственных средств |
| spelling | doaj.art-4ba17a4156d648f9bd42e4a9250474702025-03-02T09:53:19ZrusLLC Center of Pharmaceutical Analytics (LLC «CPHA»)Разработка и регистрация лекарственных средств2305-20662658-50492019-01-0101190194240DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR QUANTIFICATION OF PINOLINE AND ITS METABOLITE 6-OH-1,2,3,4-TETRAHYDROCARBOLINE FOR THE PURPOSE OF CYPD26 ACTIVITY DETERMINATIONR. K. Abdrashitov0A. E. Petukhov1V. V. Smirnov2I.M. Sechenov First Moscow State Medical UniversityI.M. Sechenov First Moscow State Medical University; National Research Center Institute of Immunology Federal Medical Biological Agency of RussiaI.M. Sechenov First Moscow State Medical University; National Research Center Institute of Immunology Federal Medical Biological Agency of RussiaA sensitive method for quantification of pinoline and its metabolite in human plasma using solid phase extraction and high performance liquid chromatography with mass-spectrometry was developed. Analytical range was 250-1500 pg/ml both for pinoline and for 6-hydroxy-1,2,3,4-tetrahydro-β-carboline. The developed method may be used to determine the current status of CYP2D6 activity.https://www.pharmjournal.ru/jour/article/view/240cyp2d6lc-ms/mspinoline6-hydroxy-1,2,3,4-tetrahydro-β-carboline |
| spellingShingle | R. K. Abdrashitov A. E. Petukhov V. V. Smirnov DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR QUANTIFICATION OF PINOLINE AND ITS METABOLITE 6-OH-1,2,3,4-TETRAHYDROCARBOLINE FOR THE PURPOSE OF CYPD26 ACTIVITY DETERMINATION Разработка и регистрация лекарственных средств cyp2d6 lc-ms/ms pinoline 6-hydroxy-1,2,3,4-tetrahydro-β-carboline |
| title | DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR QUANTIFICATION OF PINOLINE AND ITS METABOLITE 6-OH-1,2,3,4-TETRAHYDROCARBOLINE FOR THE PURPOSE OF CYPD26 ACTIVITY DETERMINATION |
| title_full | DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR QUANTIFICATION OF PINOLINE AND ITS METABOLITE 6-OH-1,2,3,4-TETRAHYDROCARBOLINE FOR THE PURPOSE OF CYPD26 ACTIVITY DETERMINATION |
| title_fullStr | DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR QUANTIFICATION OF PINOLINE AND ITS METABOLITE 6-OH-1,2,3,4-TETRAHYDROCARBOLINE FOR THE PURPOSE OF CYPD26 ACTIVITY DETERMINATION |
| title_full_unstemmed | DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR QUANTIFICATION OF PINOLINE AND ITS METABOLITE 6-OH-1,2,3,4-TETRAHYDROCARBOLINE FOR THE PURPOSE OF CYPD26 ACTIVITY DETERMINATION |
| title_short | DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR QUANTIFICATION OF PINOLINE AND ITS METABOLITE 6-OH-1,2,3,4-TETRAHYDROCARBOLINE FOR THE PURPOSE OF CYPD26 ACTIVITY DETERMINATION |
| title_sort | development and validation of bioanalytical method for quantification of pinoline and its metabolite 6 oh 1 2 3 4 tetrahydrocarboline for the purpose of cypd26 activity determination |
| topic | cyp2d6 lc-ms/ms pinoline 6-hydroxy-1,2,3,4-tetrahydro-β-carboline |
| url | https://www.pharmjournal.ru/jour/article/view/240 |
| work_keys_str_mv | AT rkabdrashitov developmentandvalidationofbioanalyticalmethodforquantificationofpinolineanditsmetabolite6oh1234tetrahydrocarbolineforthepurposeofcypd26activitydetermination AT aepetukhov developmentandvalidationofbioanalyticalmethodforquantificationofpinolineanditsmetabolite6oh1234tetrahydrocarbolineforthepurposeofcypd26activitydetermination AT vvsmirnov developmentandvalidationofbioanalyticalmethodforquantificationofpinolineanditsmetabolite6oh1234tetrahydrocarbolineforthepurposeofcypd26activitydetermination |