Genome‐scale engineering for systems and synthetic biology
Genome‐modification technologies enable the rational engineering and perturbation of biological systems. Historically, these methods have been limited to gene insertions or mutations at random or at a few pre‐defined locations across the genome. The handful of methods capable of targeted gene editin...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2013-01-01
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| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/msb.2012.66 |
| _version_ | 1797280235837718528 |
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| author | Kevin M Esvelt Harris H Wang |
| author_facet | Kevin M Esvelt Harris H Wang |
| author_sort | Kevin M Esvelt |
| collection | DOAJ |
| description | Genome‐modification technologies enable the rational engineering and perturbation of biological systems. Historically, these methods have been limited to gene insertions or mutations at random or at a few pre‐defined locations across the genome. The handful of methods capable of targeted gene editing suffered from low efficiencies, significant labor costs, or both. Recent advances have dramatically expanded our ability to engineer cells in a directed and combinatorial manner. Here, we review current technologies and methodologies for genome‐scale engineering, discuss the prospects for extending efficient genome modification to new hosts, and explore the implications of continued advances toward the development of flexibly programmable chasses, novel biochemistries, and safer organismal and ecological engineering. |
| first_indexed | 2024-03-07T16:37:18Z |
| format | Article |
| id | doaj.art-4ba1add0595b4f5a8e934ce97704b234 |
| institution | Directory Open Access Journal |
| issn | 1744-4292 |
| language | English |
| last_indexed | 2024-03-07T16:37:18Z |
| publishDate | 2013-01-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj.art-4ba1add0595b4f5a8e934ce97704b2342024-03-03T09:32:04ZengSpringer NatureMolecular Systems Biology1744-42922013-01-0191n/an/a10.1038/msb.2012.66Genome‐scale engineering for systems and synthetic biologyKevin M Esvelt0Harris H Wang1Wyss Institute for Biologically Inspired Engineering, Harvard University Boston MA USAWyss Institute for Biologically Inspired Engineering, Harvard University Boston MA USAGenome‐modification technologies enable the rational engineering and perturbation of biological systems. Historically, these methods have been limited to gene insertions or mutations at random or at a few pre‐defined locations across the genome. The handful of methods capable of targeted gene editing suffered from low efficiencies, significant labor costs, or both. Recent advances have dramatically expanded our ability to engineer cells in a directed and combinatorial manner. Here, we review current technologies and methodologies for genome‐scale engineering, discuss the prospects for extending efficient genome modification to new hosts, and explore the implications of continued advances toward the development of flexibly programmable chasses, novel biochemistries, and safer organismal and ecological engineering.https://doi.org/10.1038/msb.2012.66directed evolutiongenome engineeringmetabolic engineeringsynthesissynthetic chassis |
| spellingShingle | Kevin M Esvelt Harris H Wang Genome‐scale engineering for systems and synthetic biology Molecular Systems Biology directed evolution genome engineering metabolic engineering synthesis synthetic chassis |
| title | Genome‐scale engineering for systems and synthetic biology |
| title_full | Genome‐scale engineering for systems and synthetic biology |
| title_fullStr | Genome‐scale engineering for systems and synthetic biology |
| title_full_unstemmed | Genome‐scale engineering for systems and synthetic biology |
| title_short | Genome‐scale engineering for systems and synthetic biology |
| title_sort | genome scale engineering for systems and synthetic biology |
| topic | directed evolution genome engineering metabolic engineering synthesis synthetic chassis |
| url | https://doi.org/10.1038/msb.2012.66 |
| work_keys_str_mv | AT kevinmesvelt genomescaleengineeringforsystemsandsyntheticbiology AT harrishwang genomescaleengineeringforsystemsandsyntheticbiology |