qPCR multiplex detection of microRNA and messenger RNA in a single reaction

Reverse Transcription-Quantitative PCR (RT-qPCR) is one of the standards for analytical measurement of different RNA species in biological models. However, current Reverse Transcription (RT) based priming strategies are unable to synthesize differing RNAs and ncRNAs especially miRNAs, within a singl...

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Bibliographic Details
Main Authors: Samantha Khoury, Nham Tran
Format: Article
Language:English
Published: PeerJ Inc. 2020-06-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/9004.pdf