qPCR multiplex detection of microRNA and messenger RNA in a single reaction
Reverse Transcription-Quantitative PCR (RT-qPCR) is one of the standards for analytical measurement of different RNA species in biological models. However, current Reverse Transcription (RT) based priming strategies are unable to synthesize differing RNAs and ncRNAs especially miRNAs, within a singl...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
PeerJ Inc.
2020-06-01
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Series: | PeerJ |
Subjects: | |
Online Access: | https://peerj.com/articles/9004.pdf |