Isolation of HDL by sequential flotation ultracentrifugation followed by size exclusion chromatography reveals size-based enrichment of HDL-associated proteins

Abstract High-density lipoprotein (HDL) particles have multiple beneficial and cardioprotective roles, yet our understanding of their full structural and functional repertoire is limited due to challenges in separating HDL particles from contaminating plasma proteins and other lipid-carrying particl...

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Bibliographic Details
Main Authors: Jack Jingyuan Zheng, Joanne K. Agus, Brian V. Hong, Xinyu Tang, Christopher H. Rhodes, Hannah E. Houts, Chenghao Zhu, Jea Woo Kang, Maurice Wong, Yixuan Xie, Carlito B. Lebrilla, Emily Mallick, Kenneth W. Witwer, Angela M. Zivkovic
Format: Article
Language:English
Published: Nature Portfolio 2021-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-95451-3