The microbial community in a high-temperature enhanced biological phosphorus removal (EBPR) process
An enhanced biological phosphorus removal (EBPR) process operated at a relatively high temperature, 28 °C, removed 85% carbon and 99% phosphorus from wastewater over a period of two years. This study investigated its microbial community through fluorescent in situ hybridization (FISH) and clone libr...
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| Format: | Article |
| Language: | English |
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BMC
2016-01-01
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| Series: | Sustainable Environment Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468203916300073 |
| _version_ | 1828524826336165888 |
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| author | Ying Hui Ong Adeline Seak May Chua Yu Tzu Huang Gek Cheng Ngoh Sheng Jie You |
| author_facet | Ying Hui Ong Adeline Seak May Chua Yu Tzu Huang Gek Cheng Ngoh Sheng Jie You |
| author_sort | Ying Hui Ong |
| collection | DOAJ |
| description | An enhanced biological phosphorus removal (EBPR) process operated at a relatively high temperature, 28 °C, removed 85% carbon and 99% phosphorus from wastewater over a period of two years. This study investigated its microbial community through fluorescent in situ hybridization (FISH) and clone library generation. Through FISH, considerably more Candidatus “Accumulibacter phosphatis” (Accumulibacter)-polyphosphate accumulating organisms (PAOs) than Candidatus ‘Competibacter phosphatis’ (Competibacter)-glycogen accumulating organisms were detected in the reactor, at 36 and 7% of total bacterial population, respectively. A low ratio of Glycogen/Volatile Fatty Acid of 0.69 further indicated the dominance of PAOs in the reactor. From clone library generated, 26 operational taxonomy units were retrieved from the sludge and a diverse population was shown, comprising Proteobacteria (69.6%), Actinobacteria (13.7%), Bacteroidetes (9.8%), Firmicutes (2.94%), Planctomycetes (1.96%), and Acidobacteria (1.47%). Accumulibacter are the only recognized PAOs revealed by the clone library. Both the clone library and FISH results strongly suggest that Accumulibacter are the major PAOs responsible for the phosphorus removal in this long-term EBPR at relatively high temperature. |
| first_indexed | 2024-12-11T20:54:53Z |
| format | Article |
| id | doaj.art-4c03d4c8d84342858814e1438775dece |
| institution | Directory Open Access Journal |
| issn | 2468-2039 |
| language | English |
| last_indexed | 2024-12-11T20:54:53Z |
| publishDate | 2016-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Sustainable Environment Research |
| spelling | doaj.art-4c03d4c8d84342858814e1438775dece2022-12-22T00:51:08ZengBMCSustainable Environment Research2468-20392016-01-01261141910.1016/j.serj.2016.04.001The microbial community in a high-temperature enhanced biological phosphorus removal (EBPR) processYing Hui Ong0Adeline Seak May Chua1Yu Tzu Huang2Gek Cheng Ngoh3Sheng Jie You4Department of Chemical Engineering, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Chemical Engineering, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Bioenvironmental Engineering, Chung Yuan Christian University, Taoyuan City 32023, TaiwanDepartment of Chemical Engineering, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Bioenvironmental Engineering, Chung Yuan Christian University, Taoyuan City 32023, TaiwanAn enhanced biological phosphorus removal (EBPR) process operated at a relatively high temperature, 28 °C, removed 85% carbon and 99% phosphorus from wastewater over a period of two years. This study investigated its microbial community through fluorescent in situ hybridization (FISH) and clone library generation. Through FISH, considerably more Candidatus “Accumulibacter phosphatis” (Accumulibacter)-polyphosphate accumulating organisms (PAOs) than Candidatus ‘Competibacter phosphatis’ (Competibacter)-glycogen accumulating organisms were detected in the reactor, at 36 and 7% of total bacterial population, respectively. A low ratio of Glycogen/Volatile Fatty Acid of 0.69 further indicated the dominance of PAOs in the reactor. From clone library generated, 26 operational taxonomy units were retrieved from the sludge and a diverse population was shown, comprising Proteobacteria (69.6%), Actinobacteria (13.7%), Bacteroidetes (9.8%), Firmicutes (2.94%), Planctomycetes (1.96%), and Acidobacteria (1.47%). Accumulibacter are the only recognized PAOs revealed by the clone library. Both the clone library and FISH results strongly suggest that Accumulibacter are the major PAOs responsible for the phosphorus removal in this long-term EBPR at relatively high temperature.http://www.sciencedirect.com/science/article/pii/S2468203916300073Biological phosphorus removalTemperaturePolyphosphate accumulating organismsAccumulibacterCompetibacter |
| spellingShingle | Ying Hui Ong Adeline Seak May Chua Yu Tzu Huang Gek Cheng Ngoh Sheng Jie You The microbial community in a high-temperature enhanced biological phosphorus removal (EBPR) process Sustainable Environment Research Biological phosphorus removal Temperature Polyphosphate accumulating organisms Accumulibacter Competibacter |
| title | The microbial community in a high-temperature enhanced biological phosphorus removal (EBPR) process |
| title_full | The microbial community in a high-temperature enhanced biological phosphorus removal (EBPR) process |
| title_fullStr | The microbial community in a high-temperature enhanced biological phosphorus removal (EBPR) process |
| title_full_unstemmed | The microbial community in a high-temperature enhanced biological phosphorus removal (EBPR) process |
| title_short | The microbial community in a high-temperature enhanced biological phosphorus removal (EBPR) process |
| title_sort | microbial community in a high temperature enhanced biological phosphorus removal ebpr process |
| topic | Biological phosphorus removal Temperature Polyphosphate accumulating organisms Accumulibacter Competibacter |
| url | http://www.sciencedirect.com/science/article/pii/S2468203916300073 |
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