Two-step derivatization for determination of sugar phosphates in plants by combined reversed phase chromatography/tandem mass spectrometry

Abstract Background Sugar phosphates are important intermediates of central carbon metabolism in biological systems, with roles in glycolysis, the pentose–phosphate pathway, tricarboxylic acid (TCA) cycle, and many other biosynthesis pathways. Understanding central carbon metabolism requires a simpl...

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Bibliographic Details
Main Authors: Umut Rende, Totte Niittylä, Thomas Moritz
Format: Article
Language:English
Published: BMC 2019-11-01
Series:Plant Methods
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13007-019-0514-9