Interkit Reproducibility of the Indirect Immunofluorescence Assay on HEp-2 Cells Depends on the Immunofluorescence Reactivity Intensity and Pattern

Interkit Reproducibility of the Indirect Immunofluorescence Assay on HEp-2 Cells Depends on the Immunofluorescence Reactivity Intensity and Pattern

IntroductionThe indirect immunofluorescence assay on HEp-2 cells (HEp-2/IFA) is used worldwide for screening for autoantibodies to cellular antigens. Cell culture and fixation methods influence the cell distribution of autoantigens and the preservation of epitopes. Therefore, discrepancy of results...

Full description

Bibliographic Details
Main Authors: Mônica Jesus Silva, Alessandra Dellavance, Danielle Cristiane Baldo, Silvia Helena Rodrigues, Marcelle Grecco, Monica Simon Prado, Renan Agustinelli, Luís Eduardo Coelho Andrade
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Immunology
Subjects:
autoantibody
antinuclear antibodies
immunofluorescence
HEp-2 cells
autoimmune diseases
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2021.798322/full

Internet

https://www.frontiersin.org/articles/10.3389/fimmu.2021.798322/full

Similar Items