CRISPR-Cas9 Targeting of Hepatitis B Virus Covalently Closed Circular DNA Generates Transcriptionally Active Episomal Variants

CRISPR-Cas9 Targeting of Hepatitis B Virus Covalently Closed Circular DNA Generates Transcriptionally Active Episomal Variants

ABSTRACT Chronic hepatitis B virus (HBV) infection persists due to the lack of therapies that effectively target the HBV covalently closed circular DNA (cccDNA). We used HBV-specific guide RNAs (gRNAs) and CRISPR-Cas9 and determined the fate of cccDNA after gene editing. We set up a ribonucleoprotei...

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Bibliographic Details
Main Authors: Maria Guadalupe Martinez, Emmanuel Combe, Aurore Inchauspe, Philippe Emmanuel Mangeot, Elodie Delberghe, Fleur Chapus, Gregory Neveu, Antoine Alam, Kara Carter, Barbara Testoni, Fabien Zoulim
Format: Article
Language:English
Published: American Society for Microbiology 2022-04-01
Series:mBio
Subjects:
hepatitis B virus
covalently closed circular DNA
CRISPR-Cas9
Online Access:https://journals.asm.org/doi/10.1128/mbio.02888-21

Internet

https://journals.asm.org/doi/10.1128/mbio.02888-21

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