Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast

Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast

Abstract Ubiquitin‐protein ligases (E3s) are responsible for target recognition and regulate stability, localization or function of their substrates. However, the substrates of most E3 enzymes remain unknown. Here, we describe the development of a novel proteomic in vitro ubiquitination screen using...

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Bibliographic Details
Main Authors: Ronish Gupta, Bart Kus, Christopher Fladd, James Wasmuth, Raffi Tonikian, Sachdev Sidhu, Nevan J Krogan, John Parkinson, Daniela Rotin
Format: Article
Language:English
Published: Springer Nature 2007-06-01
Series:Molecular Systems Biology
Subjects:
endocytosis
Nedd4
post‐translational modification
proteomics
ubiquitin ligase
Online Access:https://doi.org/10.1038/msb4100159

Internet

https://doi.org/10.1038/msb4100159

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