Structural study of the asparagine-linked oligosaccharides of lipophorin in locusts
The complete structure of oligosaccharides from locust lipophorin was studied. The asparagine-linked oligosaccharides were first liberated from the protein moiety of lipophorin by digestion with almond glycopeptidase (N-oligosaccharide glycopeptidase, EC 3.5.1.52). Two major oligosaccharides (E and...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
1987-04-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752038696X |
| _version_ | 1828939894155640832 |
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| author | E Nagao H Chino |
| author_facet | E Nagao H Chino |
| author_sort | E Nagao |
| collection | DOAJ |
| description | The complete structure of oligosaccharides from locust lipophorin was studied. The asparagine-linked oligosaccharides were first liberated from the protein moiety of lipophorin by digestion with almond glycopeptidase (N-oligosaccharide glycopeptidase, EC 3.5.1.52). Two major oligosaccharides (E and F), separated by subsequent thin-layer chromatography, were analyzed by methylation analysis and 1H-NMR. Based on the experimental data, the whole structure of oligosaccharide E was identified as Man alpha 1----2Man alpha 1----6(Man alpha 1----2Man alpha 1----3) Man alpha 1----6(Man alpha 1----2Man alpha 1----2Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4GlcNAc. The data also revealed that oligosaccharide F is identical with oligosaccharide E in the structure, except for one glucose residue that is linked to the nonreducing terminal Man alpha 1----2 residue. |
| first_indexed | 2024-12-14T03:09:52Z |
| format | Article |
| id | doaj.art-4c86c547eafe4e8fa72cc68e663642d8 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T03:09:52Z |
| publishDate | 1987-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-4c86c547eafe4e8fa72cc68e663642d82022-12-21T23:19:17ZengElsevierJournal of Lipid Research0022-22751987-04-01284450454Structural study of the asparagine-linked oligosaccharides of lipophorin in locustsE NagaoH ChinoThe complete structure of oligosaccharides from locust lipophorin was studied. The asparagine-linked oligosaccharides were first liberated from the protein moiety of lipophorin by digestion with almond glycopeptidase (N-oligosaccharide glycopeptidase, EC 3.5.1.52). Two major oligosaccharides (E and F), separated by subsequent thin-layer chromatography, were analyzed by methylation analysis and 1H-NMR. Based on the experimental data, the whole structure of oligosaccharide E was identified as Man alpha 1----2Man alpha 1----6(Man alpha 1----2Man alpha 1----3) Man alpha 1----6(Man alpha 1----2Man alpha 1----2Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4GlcNAc. The data also revealed that oligosaccharide F is identical with oligosaccharide E in the structure, except for one glucose residue that is linked to the nonreducing terminal Man alpha 1----2 residue.http://www.sciencedirect.com/science/article/pii/S002222752038696X |
| spellingShingle | E Nagao H Chino Structural study of the asparagine-linked oligosaccharides of lipophorin in locusts Journal of Lipid Research |
| title | Structural study of the asparagine-linked oligosaccharides of lipophorin in locusts |
| title_full | Structural study of the asparagine-linked oligosaccharides of lipophorin in locusts |
| title_fullStr | Structural study of the asparagine-linked oligosaccharides of lipophorin in locusts |
| title_full_unstemmed | Structural study of the asparagine-linked oligosaccharides of lipophorin in locusts |
| title_short | Structural study of the asparagine-linked oligosaccharides of lipophorin in locusts |
| title_sort | structural study of the asparagine linked oligosaccharides of lipophorin in locusts |
| url | http://www.sciencedirect.com/science/article/pii/S002222752038696X |
| work_keys_str_mv | AT enagao structuralstudyoftheasparaginelinkedoligosaccharidesoflipophorininlocusts AT hchino structuralstudyoftheasparaginelinkedoligosaccharidesoflipophorininlocusts |