An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from Cupriavidus necator in Escherichia coli.
Expression of multiple heterologous genes in a dedicated host is a prerequisite for approaches in synthetic biology, spanning from the production of recombinant multiprotein complexes to the transfer of tailor-made metabolic pathways. Such attempts are often exacerbated, due in most cases to a lack...
| मुख्य लेखकों: | , , , , , |
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| स्वरूप: | लेख |
| भाषा: | English |
| प्रकाशित: |
Public Library of Science (PLoS)
2013-01-01
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| श्रृंखला: | PLoS ONE |
| ऑनलाइन पहुंच: | http://europepmc.org/articles/PMC3702609?pdf=render |